| Mannitol-facilitated perfusion staining with 2,3,5-triphenyltetrazolium chloride (TTC) for detection of experimental cerebral infarction and biochemical analysis. | |
| | |
MedLine Citation:
|
PMID: 21982741 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A simple method to quantify cerebral infarction has great value for mechanistic and therapeutic studies in experimental stroke research. Immersion staining of unfixed brain slices with 2,3,5-triphenyltetrazolium chloride (TTC) is a popular method to determine cerebral infarction in preclinical studies. However, it is often difficult to apply immersion TTC-labeling to severely injured or soft newborn brains in rodents. Here we report an in vivo TTC perfusion-labeling method based on osmotic opening of blood-brain-barrier with mannitol-pretreatment. This new method delineates cortical infarction correlated with the boundary of morphological cell injury, differentiates the induction or subcellular redistribution of apoptosis-related factors between viable and damaged areas, and easily determines the size of cerebral infarction in both adult and newborn mice. Using this method, we confirmed that administration of lipopolysaccharide 72 h before hypoxia-ischemia increases the damage in neonatal mouse brains, in contrast to its effect of protective preconditioning in adults. These results demonstrate a fast and inexpensive method that simplifies the task of quantifying cerebral infarction in small or severely injured brains and assists biochemical analysis of experimental cerebral ischemia. |
| | |
Authors:
|
Yu-Yo Sun; Dianer Yang; Chia-Yi Kuan |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-10-01 |
Journal Detail:
|
Title: Journal of neuroscience methods Volume: 203 ISSN: 1872-678X ISO Abbreviation: J. Neurosci. Methods Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2011-11-21 Completed Date: 2012-03-08 Revised Date: 2013-02-21 |
Medline Journal Info:
|
Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 122-9 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
|
Division of Developmental Biology, Division of Neurology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood-Brain Barrier / drug effects Cerebral Infarction / pathology* Coloring Agents / diagnostic use* Disease Models, Animal Immunoblotting Immunohistochemistry Male Mannitol / pharmacology* Mice Perfusion Reverse Transcriptase Polymerase Chain Reaction Staining and Labeling / methods* Tetrazolium Salts / diagnostic use* |
| Grant Support | |
ID/Acronym/Agency:
|
NS 074559/NS/NINDS NIH HHS; R21 NS074559-01/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Coloring Agents; 0/Tetrazolium Salts; 69-65-8/Mannitol; 7OL20RET2I/triphenyltetrazolium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effects of apparatus design and test procedure on learning and memory performance of C57BL/6J mi...
Next Document: Plac8 Is an Inducer of C/EBP? Required for Brown Fat Differentiation, Thermoregulation, and Control ...