Document Detail


Mannitol-facilitated perfusion staining with 2,3,5-triphenyltetrazolium chloride (TTC) for detection of experimental cerebral infarction and biochemical analysis.
MedLine Citation:
PMID:  21982741     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple method to quantify cerebral infarction has great value for mechanistic and therapeutic studies in experimental stroke research. Immersion staining of unfixed brain slices with 2,3,5-triphenyltetrazolium chloride (TTC) is a popular method to determine cerebral infarction in preclinical studies. However, it is often difficult to apply immersion TTC-labeling to severely injured or soft newborn brains in rodents. Here we report an in vivo TTC perfusion-labeling method based on osmotic opening of blood-brain-barrier with mannitol-pretreatment. This new method delineates cortical infarction correlated with the boundary of morphological cell injury, differentiates the induction or subcellular redistribution of apoptosis-related factors between viable and damaged areas, and easily determines the size of cerebral infarction in both adult and newborn mice. Using this method, we confirmed that administration of lipopolysaccharide 72 h before hypoxia-ischemia increases the damage in neonatal mouse brains, in contrast to its effect of protective preconditioning in adults. These results demonstrate a fast and inexpensive method that simplifies the task of quantifying cerebral infarction in small or severely injured brains and assists biochemical analysis of experimental cerebral ischemia.
Authors:
Yu-Yo Sun; Dianer Yang; Chia-Yi Kuan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-01
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  203     ISSN:  1872-678X     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-11-21     Completed Date:  2012-03-08     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  122-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood-Brain Barrier / drug effects
Cerebral Infarction / pathology*
Coloring Agents / diagnostic use*
Disease Models, Animal
Immunoblotting
Immunohistochemistry
Male
Mannitol / pharmacology*
Mice
Perfusion
Reverse Transcriptase Polymerase Chain Reaction
Staining and Labeling / methods*
Tetrazolium Salts / diagnostic use*
Grant Support
ID/Acronym/Agency:
NS 074559/NS/NINDS NIH HHS; R21 NS074559/NS/NINDS NIH HHS; R21 NS074559-01/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Tetrazolium Salts; 3OWL53L36A/Mannitol; 7OL20RET2I/triphenyltetrazolium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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