| Manipulations in hydrogel degradation behavior enhance osteoblast function and mineralized tissue formation. | |
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MedLine Citation:
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PMID: 16846361 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydrogels were prepared by copolymerizing a degradable macromer, poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) endcapped with methacrylate groups (PEG-LA-DM), with a nondegradable macromer, poly(ethylene glycol) dimethacrylate (PEGDM). Copolymer networks consisted of 100:0, 83:17, 67:33, and 50:50 PEGDM:PEG-LA-DM mass%, essentially creating scaffolds that exhibit 0, 17, 33, and 50% degradation over the time course of the experiment. Osteoblasts were photoencapsulated in these copolymer hydrogels and cultured for 3 weeks in vitro. Metabolic activity, proliferation, and alkaline phosphatase production were enhanced by an increase PEG-LADM content and corresponding degradation. Gene expression of the cultured osteoblasts, normalized to beta-actin, was analyzed, and osteopontin and collagen type I gene expression increased with degradation. Finally, as a measure of mineralized tissue formation, calcium and phosphate deposition was analyzed biochemically and histologically. Mineralization increased with increasing concentration of PEG-LA-DM and biochemically resembled that of hydroxyapatite. |
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Authors:
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Danielle S W Benoit; Andrew R Durney; Kristi S Anseth |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Tissue engineering Volume: 12 ISSN: 1076-3279 ISO Abbreviation: Tissue Eng. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-07-18 Completed Date: 2006-09-29 Revised Date: 2011-10-25 |
Medline Journal Info:
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Nlm Unique ID: 9505538 Medline TA: Tissue Eng Country: United States |
Other Details:
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Languages: eng Pagination: 1663-73 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309-0424, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Biocompatible Materials / metabolism* Cells, Cultured Extracellular Matrix / metabolism* Hydrogels / metabolism* Osteoblasts / physiology* Rats |
| Grant Support | |
ID/Acronym/Agency:
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DE016523/DE/NIDCR NIH HHS; R01 DE016523-01/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Hydrogels |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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