Document Detail

Manipulation of the phospholipid composition of tissue culture cells.
MedLine Citation:
PMID:  4530286     Owner:  NLM     Status:  MEDLINE    
Methods have been devised to significantly alter the phospholipid composition of LM cells grown in serum-free tissue culture medium. The polar head groups of the phospholipids, as well as the acyl groups of these lipids, can be changed. When LM cells were grown in medium containing choline analogues, either N-methyl-ethanolamine or N,N-dimethylethanolamine, or the unnatural analogues, 1-2-amino-1-butanol or 3-amino-1-propanol, up to 50% of the cellular phospholipids contained the analogue supplied. When linoleate was added to the cells as a bovine serum albumin complex, up to 40% of the fatty acids of the phospholipids were linoleate. Under the conditions discussed, the polar head group composition, the fatty acid composition, or both together could be varied in the membrane phospholipids.
M Glaser; K A Ferguson; P R Vagelos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  71     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1974 Oct 
Date Detail:
Created Date:  1975-02-06     Completed Date:  1975-02-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4072-6     Citation Subset:  IM    
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MeSH Terms
Cell Membrane / metabolism
Cells, Cultured / metabolism*
Choline / analogs & derivatives,  metabolism
Chromatography, Thin Layer
Culture Media
Fatty Acids / analysis
L Cells (Cell Line)
Linoleic Acids / metabolism
Phosphatidylethanolamines / biosynthesis
Phospholipids / analysis,  metabolism*
Phosphorus Radioisotopes
Reg. No./Substance:
0/Culture Media; 0/Fatty Acids; 0/Linoleic Acids; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Phosphorus Radioisotopes; 62-49-7/Choline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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