| Manipulation of the phospholipid composition of tissue culture cells. | |
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MedLine Citation:
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PMID: 4530286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methods have been devised to significantly alter the phospholipid composition of LM cells grown in serum-free tissue culture medium. The polar head groups of the phospholipids, as well as the acyl groups of these lipids, can be changed. When LM cells were grown in medium containing choline analogues, either N-methyl-ethanolamine or N,N-dimethylethanolamine, or the unnatural analogues, 1-2-amino-1-butanol or 3-amino-1-propanol, up to 50% of the cellular phospholipids contained the analogue supplied. When linoleate was added to the cells as a bovine serum albumin complex, up to 40% of the fatty acids of the phospholipids were linoleate. Under the conditions discussed, the polar head group composition, the fatty acid composition, or both together could be varied in the membrane phospholipids. |
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Authors:
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M Glaser; K A Ferguson; P R Vagelos |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 71 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1974 Oct |
Date Detail:
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Created Date: 1975-02-06 Completed Date: 1975-02-06 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4072-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / metabolism Cells, Cultured / metabolism* Choline / analogs & derivatives, metabolism Chromatography, Thin Layer Culture Media Fatty Acids / analysis L Cells (Cell Line) Linoleic Acids / metabolism Mice Phosphatidylethanolamines / biosynthesis Phospholipids / analysis, metabolism* Phosphorus Radioisotopes |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids; 0/Linoleic Acids; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Phosphorus Radioisotopes; 62-49-7/Choline |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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