Document Detail


Manipulation of the fate of long chain polyunsaturated fatty acids in cultured cells.
MedLine Citation:
PMID:  9250604     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have studied the biosynthesis of long chain polyunsaturated fatty acids (LC-PUFA) from their precursors in cultured cells undergoing physiological modifications, or under the influence of lipid-lowering drugs or ethanol. The formation of arachidonic acid (AA, 20:4 n-6) from the percursor linoleic acid (LA, 18:2 n-6) in the neuroblastoma cells SK-N-BE is enhanced at early stages of differentiation, and declines when differentiation is complete, in concomitance with maximal accumulation of AA in cell lipids. In the monocytic cells THP-1, the biosynthesis of LC-PUFA is also enhanced by treatment with the HMGCoA reductase inhibitor simvastatin (S), an effect which is reverted by mevalonate and other intermediates of cholesterol synthesis. Maximal activation of LC-PUFA synthesis by S occurs at concentrations lower than those required for maximal inhibition of cholesterol synthesis. In the hepatoma cells HepG2, ethanol decreases the biosynthesis of LC-PUFA while potentiating the incorporation of acetate into cholesterol. LC-PUFA synthesis appears thus to be modulated in the course of cell differentiation and complex interactions between LC-PUFA and cholesterol synthesis occur, as judged from data obtained through pharmacological manipulations.
Authors:
C Galli; P Risé; F Marangoni; A Petroni; F Visioli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  57     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-01     Completed Date:  1997-10-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  23-6     Citation Subset:  IM    
Affiliation:
Institute of Pharmacological Sciences, School of Pharmacy, University of Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Arachidonic Acid / metabolism
Carcinoma, Hepatocellular / metabolism
Cell Differentiation
Cell Line
Cholesterol / biosynthesis
Enzyme Inhibitors / pharmacology
Ethanol / pharmacology
Fatty Acids, Unsaturated / biosynthesis,  metabolism*
Humans
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Linoleic Acid
Linoleic Acids / metabolism
Liver Neoplasms / metabolism
Lovastatin / analogs & derivatives,  pharmacology
Mevalonic Acid / pharmacology
Monocytes / metabolism
Neuroblastoma / metabolism
Simvastatin
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Fatty Acids, Unsaturated; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 0/Linoleic Acids; 150-97-0/Mevalonic Acid; 2197-37-7/Linoleic Acid; 506-32-1/Arachidonic Acid; 57-88-5/Cholesterol; 64-17-5/Ethanol; 75330-75-5/Lovastatin; 79902-63-9/Simvastatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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