Document Detail


Manipulation of bacteriophages with dielectrophoresis on carbon nanofiber nanoelectrode arrays.
MedLine Citation:
PMID:  23348683     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work describes efficient manipulation of bacteriophage virus particles using a nanostructured DEP device. The nonuniform electric field for DEP is created by utilizing a nanoelectrode array (NEA) made of vertically aligned carbon nanofibers versus a macroscopic indium tin oxide electrode in a "points-and-lid" configuration integrated in a microfluidic channel. The capture of the virus particles has been systematically investigated versus the flow velocity, sinusoidal AC frequency, peak-to-peak voltage, and virus concentration. The DEP capture at all conditions is reversible and the captured virus particles are released immediately when the voltage is turned off. At the low virus concentration (8.9 × 10(4) pfu/mL), the DEP capture efficiency up to 60% can be obtained. The virus particles are individually captured at isolated nanoelectrode tips and accumulate linearly with time. Due to the comparable size, it is more effective to capture virus particles than larger bacterial cells with such NEA-based DEP devices. This technique can be potentially utilized as a fast sample preparation module in a microfluidic chip to capture, separate, and concentrate viruses and other biological particles in small volumes of dilute solutions in a portable detection system for field applications.
Authors:
Foram R Madiyar; Lateef U Syed; Christopher T Culbertson; Jun Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-03-11
Journal Detail:
Title:  Electrophoresis     Volume:  34     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-04     Completed Date:  2013-07-15     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1123-30     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage T4 / chemistry,  isolation & purification*
Carbon / chemistry*
Electrophoresis / instrumentation*,  methods*
Equipment Design
Microarray Analysis / instrumentation,  methods
Microelectrodes
Nanofibers / chemistry*
Nanotechnology / instrumentation*,  methods*
Particle Size
Grant Support
ID/Acronym/Agency:
R15 CA159250/CA/NCI NIH HHS; R15CA159250/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
7440-44-0/Carbon
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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