Document Detail


Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology.
MedLine Citation:
PMID:  23299847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chemically-inducible dimerization (CID) is a powerful tool that has proved useful in solving numerous problems in cell biology and related fields. In this review, we focus on case studies where CID was able to provide insight into otherwise refractory problems. Of particular interest are the cases of lipid second messengers and small GTPases, where the "signaling paradox" (how a small pool of signaling molecules can generate a large range of responses) can be at least partly explained through results gleaned from CID experiments. We also discuss several recent technical advances that provide improved specificity in CID action, novel CID substrates that allow simultaneous orthogonal manipulation of multiple systems in one cell, and several applications that move beyond the traditional CID technique of moving a protein of interest to a specific spatiotemporal location.
Authors:
Robert DeRose; Takafumi Miyamoto; Takanari Inoue
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2013-01-09
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  465     ISSN:  1432-2013     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-08-13     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  409-17     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Humans
Lipid Metabolism
Monomeric GTP-Binding Proteins / metabolism
Protein Multimerization / drug effects*
Signal Transduction*
Sirolimus / pharmacology
Tacrolimus Binding Proteins / chemistry,  drug effects
Grant Support
ID/Acronym/Agency:
GM092930/GM/NIGMS NIH HHS; R01 GM092930/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 3.6.5.2/Monomeric GTP-Binding Proteins; EC 5.2.1.-/Tacrolimus Binding Proteins; W36ZG6FT64/Sirolimus
Comments/Corrections

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