| α-Mangostin induces Apoptosis in Human Chondrosarcoma Cells through Down Regulation of ERK/JNK and Akt Signaling Pathway. | |
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MedLine Citation:
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PMID: 21446759 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Chondrosarcoma is a malignant primary bone tumor that is resistant to chemotherapy and radiation therapy. α-mangostin, a component of Garcinia mangostana Linn, is a xanthone derivative shown to have antioxidant and antitumor properties. This study is the first to investigate anticancer effects of α-mangostin in human chondrosarcoma cell line SW1353. We showed that α-mangostin inhibited cell proliferation of SW1353 cells in a time- and dose-dependent manner by using trypan blue exclusion method. Hoechst 33342 nuclear staining and nucleosomal DNA-gel electrophoresis revealed that α-mangostin could induce nuclear condensation and fragmentation, typically seen in apoptosis. Flow cytometry using Annexin V/PI double staining assessed apoptosis, necrosis and viability. α-mangostin activated caspase-3, -8, -9 expression, decreased Bcl-2 and increased Bax. This promotes mitochondrial dysfunction, leading to the release of cytochrome c from the mitochondria to the cytoplasm. In addition, total and phosphorylated ERK and JNK were down regulated in α-mangostin-treated SW1353 cells but no changes in p38. α-mangostin also decreased phosphorelated Akt without altering total Akt. These results suggest that α-mangostin inhinbited cell proliferation and induced apoptosis through down regulation of ERK, JNK and Akt signaling pathway in human chondrosarcoma SW1353 cells. |
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Authors:
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Aungkhana Krajarng; Yukio Nakamura; Sunit Suksamrarn; Ramida Watanapokasin |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-29 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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