Document Detail


Mango (Mangifera indica L.) peel extract fractions from different cultivars differentially affect lipid accumulation in 3T3-L1 adipocyte cells.
MedLine Citation:
PMID:  23295454     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Plant phytochemicals are increasingly recognised as sources of bioactive molecules which may have potential benefit in many health conditions. In mangoes, peel extracts from different cultivars exhibit varying effects on adipogenesis in the 3T3-L1 adipocyte cell line. In this study, the effects of preparative HPLC fractions of methanol peel extracts from Irwin, Nam Doc Mai and Kensington Pride mangoes were evaluated. Fraction 1 contained the most hydrophilic components while subsequent fractions contained increasingly more hydrophobic components. High content imaging was used to assess mango peel fraction effects on lipid accumulation, nuclei count and nuclear area in differentiating 3T3-L1 cells. For all three mango cultivars, the more hydrophilic peel fractions 1-3 inhibited lipid accumulation with greater potency than the more hydrophobic peel fractions 4. For all three cultivars, the more lipophilic fraction 4 had concentrations that enhanced lipid accumulation greater than fractions 1-3 as assessed by lipid droplet integrated intensity. The potency of this fraction 4 varied significantly between cultivars. Using mass spectrometry, five long chain free fatty acids were detected in fraction 4; these were not present in any other peel extract fractions. Total levels varied between cultivars, with Irwin fraction 4 containing the highest levels of these free fatty acids. Lipophilic components appear to be responsible for the lipid accumulation promoting effects of some mango extracts and are the likely cause of the diverse effects of peel extracts from different mango cultivars on lipid accumulation.
Authors:
Meng-Wong Taing; Jean-Thomas Pierson; Paul N Shaw; Ralf G Dietzgen; Sarah J Roberts-Thomson; Michael J Gidley; Gregory R Monteith
Related Documents :
23885674 - Capillary electrophoretic study of amine/carboxylic acid-functionalized carbon nanodots.
24685324 - Omega-3 fatty acids for postoperative atrial fibrillation: alone or in combination with...
24254284 - Effects of salicylic acid on growth and stomatal movements ofvicia faba l.: evidence fo...
24212734 - Investigation on the conformation change of hemoglobin immobilized on mpa-modified elec...
2322294 - Reactivity of diflunisal acyl glucuronide in human and rat plasma and albumin solutions.
6120164 - Thiazolidine-2-carboxylic acid, an adduct of cysteamine and glyoxylate, as a substrate ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-8
Journal Detail:
Title:  Food & function     Volume:  -     ISSN:  2042-650X     ISO Abbreviation:  Food Funct     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101549033     Medline TA:  Food Funct     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
School of Pharmacy, The University of Queensland, Queensland 4072, Australia. gregm@uq.edu.au.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Intra-individual changes of active matrix metalloproteinase-9 are associated with clinical in-stent ...
Next Document:  Yeast protective response to arsenate involves the repression of the high affinity iron uptake syste...