Document Detail


Mangiferin aglycone attenuates radiation-induced damage on human intestinal epithelial cells.
MedLine Citation:
PMID:  22422649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies suggest that mangiferin aglycone (norathyriol) has great potential as a novel radioprotector without any known toxic side effects. In this study, we assessed the protective effects of mangiferin aglycone against radiation-induced injuries on normal human intestinal epithelial cells (HIECs), while using mangiferin as a reference compound. The in vitro experiments showed that pretreatment of either mangiferin aglycone or mangiferin could inhibit cytotoxic effects of ionizing irradiation (IR) on HIECs. Cellular changes were estimated by measuring cell viability, clonogenic surviving rate, and apoptotic rate. Compared to mangiferin, we found mangiferin aglycone had greater radioprotective effects of mangiferin aglycone on HIECs. It has been demonstrated that the cytotoxicity of ionizing radiation relates to its capacity to induce DNA damage. In view of this, we monitored DNA double-strand breaks (DSBs) using γH2AX foci formation to test whether mangiferin aglycone and mangiferin could modulate genotoxic effects of radiation. It shows that mangiferin aglycone could eliminate 46.8% of the total DSBs of the cells exposed to 2 Gy IR, which is significantly better than mangiferin. Complementing earlier results from our group, it appears possible to conclude that mangiferin aglycone presents potential useful effects on IR-induced damage and may be a better radioprotective agent than mangiferin therapeutically.
Authors:
Jixiao Lei; Chuanfeng Zhou; Honggang Hu; Lina Hu; Meijia Zhao; Yanyong Yang; Yunhai Chuai; Jin Ni; Jianming Cai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  113     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-06-19     Completed Date:  2012-10-19     Revised Date:  2013-01-28    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2633-42     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Faculty of Naval Medicine, Department of Radiation Medicine, Second Military Medical University, Shanghai, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
DNA Damage / drug effects,  radiation effects
Epithelial Cells / drug effects*,  radiation effects*
Humans
Intestines / cytology*
Radiation, Ionizing
Xanthones / pharmacology*
Chemical
Reg. No./Substance:
0/Xanthones; 1M84LD0UMD/mangiferin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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