| Manganese enhancement in non-CNS organs. | |
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MedLine Citation:
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PMID: 20878971 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Manganese-enhanced magnetic resonance imaging (MEMRI) is a novel imaging technique capable of monitoring calcium influx, in vivo. Manganese (Mn2+) ions, similar to calcium ions (Ca2+), are taken up by activated cells where their paramagnetic properties afford signal enhancement in T(1)-weighted MRI methodologies. In this study we have assessed Mn2+ distribution in mice using magnetization-prepared rapid gradient echo (MP-RAGE) based MRI, by measuring changes in T(1)-effective relaxation times (T(1)-eff), effective R(1)-relaxation rates (R(1)-eff) and signal intensity (SI) profiles over time. The manganese concentration in the tissue was also determined using inductively coupled plasma atomic emission spectrometry (ICP-AES). Our results show a strong positive correlation between infused dose of MnCl2 and the tissue manganese concentration. Furthermore, we demonstrate a linear relationship between R(1)-eff and tissue manganese concentration and tissue-specific Mn2+ distribution in murine tissues following dose-dependent Mn2+ administration. This data provides an optimized MnCl2 dose regimen for an MP-RAGE based sequence protocol for specific target organs and presents a potential 3D MRI technique for in vivo imaging of Ca2+ entry during Ca2+-dependent processes in a wide range of tissues. |
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Authors:
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Li-Wen Lee; Po-Wah So; Anthony N Price; James R C Parkinson; David J Larkman; Jane Halliday; Simon M Poucher; John A T Pugh; Alan G Cox; Cameron W McLeod; Jimmy D Bell |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: NMR in biomedicine Volume: 23 ISSN: 1099-1492 ISO Abbreviation: NMR Biomed Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-29 Completed Date: 2011-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8915233 Medline TA: NMR Biomed Country: England |
Other Details:
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Languages: eng Pagination: 931-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
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Metabolic and Molecular Imaging Group, MRC Clinical Sciences Centre, Hammersmith Hospital Campus, Imperial College London, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chlorides* / administration & dosage, metabolism Image Enhancement / methods* Magnetic Resonance Imaging / methods* Male Manganese* / chemistry, metabolism Manganese Compounds* / administration & dosage, metabolism Mice Mice, Inbred C57BL Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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//Medical Research Council |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Manganese Compounds; 7439-96-5/Manganese; 7773-01-5/manganese chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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