Document Detail

Manganese effects on haematopoietic cells and circulating coelomocytes of Asterias rubens (Linnaeus).
MedLine Citation:
PMID:  18639346     Owner:  NLM     Status:  MEDLINE    
Manganese (Mn) is a naturally abundant metal in marine sediments where it mainly occurs as MnO(2). During hypoxic conditions it is converted into a bioavailable state, Mn(2+), and can reach levels that previously have shown effects on immune competent cells of the crustacean, Nephrops norvegicus. Here we investigated if Mn also affects circulating coelomocytes and their renewal in the common sea star, Asterias rubens, when exposed to concentrations of Mn that can be found in nature. When the sea stars were exposed to Mn it accumulated in the coelomic fluid and the number of circulating coelomocytes, in contrast to what was recorded in Nephrops, increased significantly. By using the substitute nucleotide, 5-bromo-2'-deoxyuridine, BrdU, for tracing cell division and by recording mitotic index by nuclei staining, we found that Mn induced proliferation of cells from a putative haematopoietic tissue, the coelomic epithelium. In addition, the haematopoietic tissue and coelomocytes showed stress response in terms of changes in HSP70 levels and protein carbonyls, as judged by immunohistochemistry and Western blotting. Measurement of dehydrogenase activity, using MTS/PMS, revealed that Mn showed cytotoxic properties. We also found that the phagocytotic capacity of coelomocytes was significantly inhibited by Mn. It was concluded that the exposure of A. rubens to Mn induced renewal of coelomocytes and impaired their immune response.
Carolina Oweson; Helen Sköld; Annalisa Pinsino; Valeria Matranga; Bodil Hernroth
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-13
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  89     ISSN:  0166-445X     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-11     Completed Date:  2009-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  75-81     Citation Subset:  IM    
Department of Marine Ecology, Göteborg University, Kristineberg 566, 45034 Fiskebäckskil, Sweden.
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MeSH Terms
Asterias / cytology,  drug effects*,  metabolism
Blotting, Western
Cell Count
Cell Proliferation / drug effects
Cell Survival / drug effects
HSP70 Heat-Shock Proteins / metabolism
Hemocytes / cytology,  drug effects*,  metabolism
Manganese / toxicity*
Mitotic Index
Phagocytosis / drug effects
Water Pollutants, Chemical / toxicity*
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Water Pollutants, Chemical; 7439-96-5/Manganese

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