| Manganese Superoxide Dismutase is not Protective in Bovine Pulmonary Artery Endothelial Cells at Systemic Oxygen Levels. | |
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MedLine Citation:
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PMID: 20874435 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Bovine pulmonary artery endothelial cells (BPAEC) are extremely sensitive to oxygen, mediated by superoxide production. Ionizing radiation is known to generate superoxide in oxygenated aqueous media; however, at systemic oxygen levels (3%), no oxygen enhancement is observed after irradiation. A number of markers (cell growth, alamarBlue, mitochondrial membrane polarization) for metabolic activity indicate that BPAEC maintained under 20% oxygen grow and metabolize more slowly than cells maintained under 3% oxygen. BPAEC cultured in 20% oxygen grow better when they are transiently transfected with either manganese superoxide dismutase (MnSOD) or copper zinc superoxide dismutase (CuZnSOD) and exhibit improved survival after irradiation (0.5–10 Gy). Furthermore, X irradiation of BPAEC grown in 20% oxygen results in very diffuse colony formation, which is completely ameliorated by either growth in 3% oxygen or overexpression of MnSOD. However, MnSOD overexpression in BPAEC grown in 3% oxygen provides no further radioprotection, as judged by clonogenic survival curves. Radiation does not increase apoptosis in BPAEC but inhibits cell growth and up-regulates p53 and p21 at either 3% or 20% oxygen. |
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Authors:
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Molly S Stitt-Fischer; Rachel K Ungerman; Daniel S Wilen; Karla Wasserloos; Lara M Renz; Shannon E Raub; Jim Peterson; Linda L Pearce |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-9-28 |
Journal Detail:
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Title: Radiation research Volume: - ISSN: 1938-5404 ISO Abbreviation: - Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-9-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401245 Medline TA: Radiat Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219-3138. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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