Document Detail

Manganese Superoxide Dismutase is not Protective in Bovine Pulmonary Artery Endothelial Cells at Systemic Oxygen Levels.
MedLine Citation:
PMID:  20874435     Owner:  NLM     Status:  Publisher    
Abstract Bovine pulmonary artery endothelial cells (BPAEC) are extremely sensitive to oxygen, mediated by superoxide production. Ionizing radiation is known to generate superoxide in oxygenated aqueous media; however, at systemic oxygen levels (3%), no oxygen enhancement is observed after irradiation. A number of markers (cell growth, alamarBlue, mitochondrial membrane polarization) for metabolic activity indicate that BPAEC maintained under 20% oxygen grow and metabolize more slowly than cells maintained under 3% oxygen. BPAEC cultured in 20% oxygen grow better when they are transiently transfected with either manganese superoxide dismutase (MnSOD) or copper zinc superoxide dismutase (CuZnSOD) and exhibit improved survival after irradiation (0.5–10 Gy). Furthermore, X irradiation of BPAEC grown in 20% oxygen results in very diffuse colony formation, which is completely ameliorated by either growth in 3% oxygen or overexpression of MnSOD. However, MnSOD overexpression in BPAEC grown in 3% oxygen provides no further radioprotection, as judged by clonogenic survival curves. Radiation does not increase apoptosis in BPAEC but inhibits cell growth and up-regulates p53 and p21 at either 3% or 20% oxygen.
Molly S Stitt-Fischer; Rachel K Ungerman; Daniel S Wilen; Karla Wasserloos; Lara M Renz; Shannon E Raub; Jim Peterson; Linda L Pearce
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-9-28
Journal Detail:
Title:  Radiation research     Volume:  -     ISSN:  1938-5404     ISO Abbreviation:  -     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-9-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219-3138.
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