Document Detail

Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae.
MedLine Citation:
PMID:  7489498     Owner:  NLM     Status:  MEDLINE    
Mammalian splicing factor SF3a consists of three subunits of 60, 66, and 120 kDa and functions early during pre-mRNA splicing by converting the U2 snRNP into its active form. A cDNA encoding the 120-kDa subunit of SF3a has been cloned. The SF3a120 gene was localized to human chromosome 22, and three mRNAs of 3.2, 3.8, and 5.7 kb are ubiquitously expressed. The N-terminal half of the deduced SF3a120 amino acid sequence contains a tandemly repeated motif (the SURP module) that has recently been identified in the essential splicing factor PRP21p of Saccharomyces cerevisiae, the Drosophila alternative splicing regulator suppressor-of-white-apricot, and four proteins from nematodes and mammals; the C-terminal half is organized into a proline-rich region and a ubiquitin-like domain. The spacing between the SURP modules and the protein's essential function in constitutive splicing identify SF3a120 as the mammalian homologue of yeast PRP21p. Binding studies with truncated derivatives of SF3a120 revealed that the SURP domains function in binding to SF3a60, whereas a region of 130 amino acids C-terminal to these domains is essential for contacts with SF3a66.
A Krämer; F Mulhauser; C Wersig; K Gröning; G Bilbe
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  RNA (New York, N.Y.)     Volume:  1     ISSN:  1355-8382     ISO Abbreviation:  RNA     Publication Date:  1995 May 
Date Detail:
Created Date:  1996-01-04     Completed Date:  1996-01-04     Revised Date:  2008-11-20    
Medline Journal Info:
Nlm Unique ID:  9509184     Medline TA:  RNA     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  260-72     Citation Subset:  IM    
Département de Biologie Cellulaire, Université de Genève, Switzerland.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 22
Cloning, Molecular
DNA, Complementary / genetics
Fungal Proteins / genetics*
Molecular Sequence Data
Multigene Family*
Protein Binding
Protein Conformation
RNA Splicing*
RNA-Binding Proteins / genetics*
Repetitive Sequences, Nucleic Acid
Ribonucleoprotein, U2 Small Nuclear*
Saccharomyces cerevisiae Proteins*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Reg. No./Substance:
0/DNA, Complementary; 0/Fungal Proteins; 0/PRP21 protein, S cerevisiae; 0/RNA-Binding Proteins; 0/Ribonucleoprotein, U2 Small Nuclear; 0/Saccharomyces cerevisiae Proteins; 0/splicing factor 3a

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