Document Detail

Mammalian proapoptotic factor ChaC1 and its homologues function as γ-glutamyl cyclotransferases acting specifically on glutathione.
MedLine Citation:
PMID:  23070364     Owner:  NLM     Status:  MEDLINE    
ChaC1 is a mammalian proapoptic protein of unknown function induced during endoplasmic reticulum stress. We show using in vivo studies and novel in vitro assays that the ChaC family of proteins function as γ-glutamyl cyclotransferases acting specifically to degrade glutathione but not other γ-glutamyl peptides. The overexpression of these proteins (but not the catalytically dead E>Q mutants) led to glutathione depletion and enhanced apoptosis in yeast. The ChaC family is conversed across all phyla and represents a new pathway for glutathione degradation in living cells, and the first cytosolic pathway for glutathione degradation in mammalian cells.
Akhilesh Kumar; Shweta Tikoo; Shuvadeep Maity; Shantanu Sengupta; Sagar Sengupta; Amandeep Kaur; Anand Kumar Bachhawat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-16
Journal Detail:
Title:  EMBO reports     Volume:  13     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-05-30     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  1095-101     Citation Subset:  IM    
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MeSH Terms
Apoptosis / genetics*
Catalytic Domain
Endoplasmic Reticulum Stress
Escherichia coli / enzymology,  genetics
Gene Expression Regulation
Glutathione* / chemistry,  metabolism
Nerve Tissue Proteins* / chemistry,  genetics,  metabolism
Peptides / metabolism
Protein Conformation
Protein Folding
Pyrrolidonecarboxylic Acid / chemistry,  metabolism
Saccharomyces cerevisiae / enzymology,  genetics
gamma-Glutamyltransferase* / chemistry,  genetics,  metabolism
Reg. No./Substance:
0/CHAC protein, mouse; 0/Nerve Tissue Proteins; 0/Peptides; EC; GAN16C9B8O/Glutathione; SZB83O1W42/Pyrrolidonecarboxylic Acid

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