Document Detail


Mammalian oocyte growth and development in vitro.
MedLine Citation:
PMID:  9115726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper is a review of the current status of technology for mammalian oocyte growth and development in vitro. It compares and contrasts the characteristics of the various culture systems that have been devised for the culture of either isolated preantral follicles or the oocyte-granulosa cell complexes form preantral follicles. The advantages and disadvantages of these various systems are discussed. Endpoints for the evaluation of oocyte development in vitro, including oocyte maturation and embryogenesis, are described. Considerations for the improvement of the culture systems are also presented. These include discussions of the possible effects of apoptosis and inappropriate differentiation of oocyte-associated granulosa cells on oocyte development. Finally, the potential applications of the technology for oocyte growth and development in vitro are discussed. For example, studies of oocyte development in vitro could help to identify specific molecules produced during oocyte development that are essential for normal early embryogenesis and perhaps recognize defects leading to infertility or abnormalities in embryonic development. Moreover, the culture systems may provide the methods necessary to enlarge the populations of valuable agricultural, pharmaceutical product-producing, and endangered animals, and to rescue the oocytes of women about to undergo clinical procedures that place oocytes at risk.
Authors:
J J Eppig; M O'Brien; K Wigglesworth
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  44     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1997-04-23     Completed Date:  1997-04-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  260-73     Citation Subset:  IM    
Affiliation:
Jackson Laboratory, Bar Harbor, Maine 04609, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division*
Cell Nucleus
Cells, Cultured
Cytoplasm
Embryonic and Fetal Development
Female
Fertilization
Humans
Mammals
Oocytes / growth & development*
Ovary / cytology
Grant Support
ID/Acronym/Agency:
CA34196/CA/NCI NIH HHS; HD21970/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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