Document Detail

Mammalian nuclear transfer.
MedLine Citation:
PMID:  16881069     Owner:  NLM     Status:  MEDLINE    
During development, the genetic content of each cell remains, with a few exceptions, identical to that of the zygote. Differentiated cells, therefore, retain all the genetic information necessary to generate an entire organism (nuclear totipotency). Nuclear transfer (NT) was initially developed to test experimentally this concept by cloning animals from differentiated cells. It has, since then, been used to study the role of genetic and epigenetic alterations during development and disease. In this review, we highlight some of the milestones in mammalian NT reached in the 50 years after the first nuclear transplantations in frogs. We also address problems associated with mammalian nuclear transfer and provide a survey on current NT and stem cell technology. In the long term, nuclear transfer or alternative strategies aim to generate customized pluripotent cells, which would be invaluable to medical research and therapy.
Alexander Meissner; Rudolf Jaenisch
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  235     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-30     Completed Date:  2006-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2460-9     Citation Subset:  IM    
Copyright Information:
Copyright 2006 Wiley-Liss, Inc.
Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
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MeSH Terms
Cloning, Organism / ethics
DNA Methylation
Epigenesis, Genetic
Nuclear Transfer Techniques*
Totipotent Stem Cells / cytology

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