Document Detail


Mammalian cell survival and processing in supercritical CO(2).
MedLine Citation:
PMID:  16651535     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrate that mammalian cells can survive for 5 min within high-pressure CO(2)(.) Cell survival was investigated by exposing a range of mammalian cell types to supercritical CO(2) (scCO(2)) (35 degrees C, 74 bar; 1 bar = 100 kPa) for increasing exposure and depressurization times. The myoblastic C2C12 cell line, 3T3 fibroblasts, chondrocytes, and hepatocytes all displayed appreciable but varying metabolic activity with exposure times up to 1 min. With depressurization times of 4 min, cell population metabolic activity was >/=70% of the control population. Based on survival data, we developed a single-step scCO(2) technique for the rapid production of biodegradable poly(dl-lactic acid) scaffolds containing mammalian cells. By using optimum cell-survival conditions, scCO(2) was used to process poly(dl-lactic acid) containing a cell suspension, and, upon pressure release, a polymer sponge containing viable mammalian cells was formed. Cell functionality was demonstrated by retention of an osteogenic response to bone morphogenetic protein-2 in C2C12 cells. A gene microarray analysis showed no statistically significant changes in gene expression across 4,418 genes by a single-class t test. A significance analysis of microarrays revealed only eight genes that were down-regulated based on a delta value of 1.0125 and a false detection rate of 0.
Authors:
Patrick J Ginty; Daniel Howard; Felicity R A J Rose; Martin J Whitaker; John J A Barry; Patrick Tighe; Stacey R Mutch; Gulay Serhatkulu; Richard O C Oreffo; Steven M Howdle; Kevin M Shakesheff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-01
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  103     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-10     Completed Date:  2006-07-19     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7426-31     Citation Subset:  IM    
Affiliation:
Schools of Pharmacy and Chemistry, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / pharmacology*
Cell Differentiation
Cell Survival / drug effects
Cells, Cultured
Gene Expression Regulation
Mice
Osteogenesis / drug effects
Sheep
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide
Comments/Corrections

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