| Mammalian cell microinjection assay to study the function of Rho family guanosine triphosphatases. | |
| | |
MedLine Citation:
|
PMID: 16878698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Microinjection is an excellent technique for studying the acute responses of cells to proteins and can be used to investigate the effects of mutations in proteins on their activity. It has been used widely to study the responses to Rho family guanosine triphosphatases and is particularly useful for cell types that are difficult to transfect. Here, we describe the procedure for microinjecting cells with purified recombinant proteins or with expression vectors encoding proteins, and for analyzing the cells after injection. |
| | |
Authors:
|
Ritu Garg; Anne J Ridley |
Related Documents
:
|
2675228 - Influence of the valency and hydrophobicity of an antigen on its efficiency of processi... 18980158 - Cellular uptake and ex vivo urothelial penetration by oligodeoxynucleotides for optimiz... 16025538 - Production and n-glycan analysis of secreted human erythropoietin glycoprotein in stabl... 17135188 - Cellular delivery of small interfering rna by a non-covalently attached cell-penetratin... 9219848 - Stromal fibroblasts influence oral squamous-cell carcinoma cell interactions with tenas... 19104058 - Dividing glial cells maintain differentiated properties including complex morphology an... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 332 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2006 |
Date Detail:
|
Created Date: 2006-08-01 Completed Date: 2006-08-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 257-67 Citation Subset: IM |
Affiliation:
|
Ludwig Institute for Cancer Research, University College London, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3T3 Cells Animals DNA / metabolism Mice Microinjections / instrumentation, methods* Recombinant Proteins / genetics, metabolism rho GTP-Binding Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Recombinant Proteins; 9007-49-2/DNA; EC 3.6.5.2/rho GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins.
Next Document: Affinity-based assay of Rho guanosine triphosphatase activation.