Document Detail


Mammalian cell microinjection assay to study the function of Rho family guanosine triphosphatases.
MedLine Citation:
PMID:  16878698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microinjection is an excellent technique for studying the acute responses of cells to proteins and can be used to investigate the effects of mutations in proteins on their activity. It has been used widely to study the responses to Rho family guanosine triphosphatases and is particularly useful for cell types that are difficult to transfect. Here, we describe the procedure for microinjecting cells with purified recombinant proteins or with expression vectors encoding proteins, and for analyzing the cells after injection.
Authors:
Ritu Garg; Anne J Ridley
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  332     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-08-01     Completed Date:  2006-08-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  257-67     Citation Subset:  IM    
Affiliation:
Ludwig Institute for Cancer Research, University College London, UK.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
DNA / metabolism
Mice
Microinjections / instrumentation,  methods*
Recombinant Proteins / genetics,  metabolism
rho GTP-Binding Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 9007-49-2/DNA; EC 3.6.5.2/rho GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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