Document Detail

Mammalian cell invasion by closely related Trypanosoma species T. dionisii and T. cruzi.
MedLine Citation:
PMID:  22079376     Owner:  NLM     Status:  Publisher    
Protozoan parasites of the genus Trypanosoma can infect virtually all mammalian species. Within this genus, Trypanosoma dionisii from bats and Trypanosoma cruzi that causes Chagas' disease, belonging to the subgenus Schizotrypanum, can invade mammalian cells. The mechanisms of cell invasion by T. dionisii are poorly understood. To address that question, metacyclic trypomastigotes (MT) and human epithelial HeLa cells were used. Similarly to genetically divergent T. cruzi strains G (TcI) and CL (TcVI), associated, respectively with marsupial and human infections, T. dionisii infectivity increased under nutritional stress, a condition that induces host cell lysosome exocytosis required for parasite internalization. For efficient internalization, T. dionisii depended on MT protein tyrosine kinase (PTK) and Ca(2+) mobilization from acidocalcisomes, whereas T. cruzi strains also relied on phosphatidylinositol 3-kinase (PI3K), protein kinase C (PKC) and Ca(2+) released from thapsigargin-sensitive compartments. T. dionisii-induced signaling in host cells implicated PKC and Ca(2+) mobilized from thapsigargin-sensitive stores, like T. cruzi, but without PI3K involvement. Unlike T. cruzi, T. dionisii metacyclic forms did not use l-proline as source of energy required for internalization. Molecules related to T. cruzi surface glycoproteins involved in MT-host cell interaction were undetectable in T. dionisii. The difference in the surface profile of the two species was also inferred from the susceptibility of T. dionisii metacyclic forms to complement-mediated lysis, as opposed to complete resistance of T. cruzi. In summary, the two Trypanosoma species display distinct surface profiles but invade host cells through a common mechanism involving lysosome mobilization to the site of parasite entry.
Fernando Yukio Maeda; Cristian Cortez; Renan Melatto Alves; Nobuko Yoshida
Related Documents :
12732976 - The role of cell hydrophobicity in the formation of aerobic granules.
17826 - In vivo and in vitro studies of nitrate reductase regulation in asperillus nidulans.
14996466 - Fermentation of banana media by using kappa-carrageenan immobilized lactobacillus acido...
6200076 - Regulation of the salvage pathway of purine nucleotide synthesis by the oxidation state...
24297096 - Melatonin protects mg63 osteoblast-like cells from hydrogen peroxide-induced cytotoxici...
22364296 - Silence of p15 expression by rnai enhances cisplatin resistance in hepatocellular carci...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-6
Journal Detail:
Title:  Acta tropica     Volume:  -     ISSN:  1873-6254     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370374     Medline TA:  Acta Trop     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, R. Pedro de Toledo, 669-6° andar, 04039-032, São Paulo, S.P., Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The subgenus Adlerius Nitzulescu (Diptera, Psychodidae, Phlebotomus) in Iran.
Next Document:  Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expressi...