Document Detail


Mammalian cell-based sensor system.
MedLine Citation:
PMID:  20091291     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Use of living cells or cellular components in biosensors is receiving increased attention and opens a whole new area of functional diagnostics. The term "mammalian cell-based biosensor" is designated to biosensors utilizing mammalian cells as the biorecognition element. Cell-based assays, such as high-throughput screening (HTS) or cytotoxicity testing, have already emerged as dependable and promising approaches to measure the functionality or toxicity of a compound (in case of HTS); or to probe the presence of pathogenic or toxigenic entities in clinical, environmental, or food samples. External stimuli or changes in cellular microenvironment sometimes perturb the "normal" physiological activities of mammalian cells, thus allowing CBBs to screen, monitor, and measure the analyte-induced changes. The advantage of CBBs is that they can report the presence or absence of active components, such as live pathogens or active toxins. In some cases, mammalian cells or plasma membranes are used as electrical capacitors and cell-cell and cell-substrate contact is measured via conductivity or electrical impedance. In addition, cytopathogenicity or cytotoxicity induced by pathogens or toxins resulting in apoptosis or necrosis could be measured via optical devices using fluorescence or luminescence. This chapter focuses mainly on the type and applications of different mammalian cell-based sensor systems.
Authors:
Pratik Banerjee; Briana Franz; Arun K Bhunia
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Advances in biochemical engineering/biotechnology     Volume:  117     ISSN:  1616-8542     ISO Abbreviation:  Adv. Biochem. Eng. Biotechnol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307733     Medline TA:  Adv Biochem Eng Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  21-55     Citation Subset:  IM    
Affiliation:
Laboratory of Food Microbiology & Immunochemistry, Department of Food & Animal Sciences, Alabama A&M University, Normal, AL, 35762, USA.
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