Document Detail


Malondialdehyde Measurement in Oxidized Foods: Evaluation of the Spectrophotometric Thiobarbituric Acid Reactive Substances (TBARS) Test in Various Foods.
MedLine Citation:
PMID:  22950760     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The ability of the spectrophotometric thiobarbituric acid reactive substances (TBARS) test to determine malondialdehyde (MDA) in various food matrices was evaluated. MDA was extracted from the foods, the extract reacted with thiobarbituric acid (TBA) and the formed TBA-MDA adduct was measured spectrophotometricaly at 352nm. In parallel, the TBA-MDA adduct was analyzed with High Performance Liquid Chromatography (HPLC) coupled with fluorescence detection. Oils, unprocessed and uncooked meat and fish products, did not exhibit any significant difference in the amount of MDA measured by the two methods , indicating that the major substance reacting with TBA and forming an adduct that absorbs at 532nm was malondialdehyde. However, in products such as dry nuts, pork sausages, cooked fish and gouda cheese an overestimation of malondialdehyde was observed, indicating TBARS test unsuitable for accurate determination of MDA. Furthermore, the results in the present work suggest that overestimation of MDA by the TBARS test as it was applied, is related to the interference of other than secondary lipid oxidation products.
Authors:
Antonios Papastergiadis; Edward Mubiru; Herman Van Langenhove; Bruno De Meulenaer
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-5
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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