Document Detail

Malignant transformation of an epithelial cell by v-Src via tv-a-mediated retroviral infection: a new cell model for studying carcinogenesis.
MedLine Citation:
PMID:  16256070     Owner:  NLM     Status:  MEDLINE    
Most human cancers are of epithelial origin, but many cell culture models for the study of cancer-causing genes use fibroblasts. In addition, efficient delivery and stable expression of foreign genes into non-transformed cell lines are often difficult. To address both questions, we here established a non-transformed rat kidney epithelial RK3E cell line that constitutively expresses tv-a (receptor for subgroup A avian leukosis virus, ALV) for delivery of foreign genes via avian retroviral infection. This cell line (RK3E/tv-a) allows efficient and stable expression of either single or multiple foreign genes. Furthermore, tv-a-mediated delivery of various oncogenes (v-src, H-ras, myc or akt) leads to malignant transformation. v-src-transformed cells exhibited classical cancerous phenotypes in vitro, and induced tumor formation and lung metastasis upon injecting into immunodeficient mice. Expression profiles of downstream molecular effectors (E-cadherin, beta-catenin, cyclin D1, Myc, VEGF, MMP-2, and MMP-9) in these cells correlate with characteristics of cancerous phenotypes. This new cell model serves as a useful tool to study cancer-causing genes in epithelial cell type.
Shu-Ling Fu; Yu-Jing Huang; Fong-Pin Liang; Yung-Fen Huang; Ching-Feng Chuang; Shih-Wei Wang; Ju-Wen Yao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-21
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  338     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-15     Completed Date:  2006-01-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  830-8     Citation Subset:  IM    
Institute of Traditional Medicine, National Yang-Ming University, 155, Sec.2, Li-Nong St., Taipei 11221, Taiwan.
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MeSH Terms
Avian Proteins / genetics,  metabolism
Cell Line, Tumor
Cell Transformation, Neoplastic / genetics,  metabolism*,  pathology*
Disease Models, Animal
Epithelial Cells / metabolism*,  pathology*
Neoplasms, Experimental / genetics,  metabolism*,  pathology*
Oncogene Protein pp60(v-src) / genetics,  metabolism*
Receptors, Virus / genetics,  metabolism
Retroviridae / genetics,  metabolism
Transfection / methods
Reg. No./Substance:
0/Avian Proteins; 0/Receptors, Virus; 0/Tva receptor; EC Protein pp60(v-src)

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