| Malignant transformation of an epithelial cell by v-Src via tv-a-mediated retroviral infection: a new cell model for studying carcinogenesis. | |
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MedLine Citation:
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PMID: 16256070 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most human cancers are of epithelial origin, but many cell culture models for the study of cancer-causing genes use fibroblasts. In addition, efficient delivery and stable expression of foreign genes into non-transformed cell lines are often difficult. To address both questions, we here established a non-transformed rat kidney epithelial RK3E cell line that constitutively expresses tv-a (receptor for subgroup A avian leukosis virus, ALV) for delivery of foreign genes via avian retroviral infection. This cell line (RK3E/tv-a) allows efficient and stable expression of either single or multiple foreign genes. Furthermore, tv-a-mediated delivery of various oncogenes (v-src, H-ras, myc or akt) leads to malignant transformation. v-src-transformed cells exhibited classical cancerous phenotypes in vitro, and induced tumor formation and lung metastasis upon injecting into immunodeficient mice. Expression profiles of downstream molecular effectors (E-cadherin, beta-catenin, cyclin D1, Myc, VEGF, MMP-2, and MMP-9) in these cells correlate with characteristics of cancerous phenotypes. This new cell model serves as a useful tool to study cancer-causing genes in epithelial cell type. |
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Authors:
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Shu-Ling Fu; Yu-Jing Huang; Fong-Pin Liang; Yung-Fen Huang; Ching-Feng Chuang; Shih-Wei Wang; Ju-Wen Yao |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-21 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 338 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-11-15 Completed Date: 2006-01-12 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 830-8 Citation Subset: IM |
Affiliation:
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Institute of Traditional Medicine, National Yang-Ming University, 155, Sec.2, Li-Nong St., Taipei 11221, Taiwan. slfu@ym.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Avian Proteins / genetics, metabolism Cell Line, Tumor Cell Transformation, Neoplastic / genetics, metabolism*, pathology* Disease Models, Animal Epithelial Cells / metabolism*, pathology* Neoplasms, Experimental / genetics, metabolism*, pathology* Oncogene Protein pp60(v-src) / genetics, metabolism* Rats Receptors, Virus / genetics, metabolism Retroviridae / genetics, metabolism Transfection / methods |
| Chemical | |
Reg. No./Substance:
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0/Avian Proteins; 0/Receptors, Virus; 0/Tva receptor; EC 2.7.10.2/Oncogene Protein pp60(v-src) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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