Document Detail


Malignancy associated changes in epithelial cells of buccal mucosa: a potential cancer detection test.
MedLine Citation:
PMID:  16447817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To analyze the presence of malignancy associated changes (MACs) in normal buccal mucosa cells of lung and breast cancer patients and their relationship to tumor subtype, stage and size. STUDY DESIGN: Buccal mucosa smears of 107 lung cancer and 100 breast cancer patients and corresponding healthy subjects were collected, stained by the DNA-specific Feulgen-thionin method and scanned using an automated high-resolution cytometer. Nuclear texture features of a minimum of 500 nuclei per slide were calculated, and statistical classifiers using Gaussian models of class-probability distribution were designed, trained and tested in 3 parts: (1) ability to separate cancer patient samples from controls, (2) cross-validation of classifiers for different cancer types, and (3) correlation of MAC expression with tumor subtype, stage and size. RESULTS: Lung and breast cancer induce MACs in normal buccal mucosa cells. The classifiers based on the selected nuclear features correctly recognized >80% of lung and breast cancer cases. The results indicate that MAC detection is not dependent on the tumor subtype, stage or size. CONCLUSION: The presence of MACs in buccal mucosa cells offers the potential for developing a new noninvasive cancer screening test.
Authors:
Marija Us-Krasovec; Janez Erzen; Mario Zganec; Margareta Strojan-Flezar; Jaka Lavrencak; David Garner; Alexei Doudkine; Branko Palcic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  27     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2006-02-01     Completed Date:  2006-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  254-62     Citation Subset:  IM    
Affiliation:
Department of Cytopathology, Institute of Oncology, Ljubljana, Slovenia. mus@onko-i.si
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Breast Neoplasms / classification,  diagnosis*,  pathology
Case-Control Studies
Epithelial Cells / pathology*
Female
Humans
Lung Neoplasms / classification,  diagnosis*,  pathology
Middle Aged
Mouth Mucosa / pathology*
Neoplasm Staging
ROC Curve

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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