| Malignancy associated changes in epithelial cells of buccal mucosa: a potential cancer detection test. | |
| | |
MedLine Citation:
|
PMID: 16447817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To analyze the presence of malignancy associated changes (MACs) in normal buccal mucosa cells of lung and breast cancer patients and their relationship to tumor subtype, stage and size. STUDY DESIGN: Buccal mucosa smears of 107 lung cancer and 100 breast cancer patients and corresponding healthy subjects were collected, stained by the DNA-specific Feulgen-thionin method and scanned using an automated high-resolution cytometer. Nuclear texture features of a minimum of 500 nuclei per slide were calculated, and statistical classifiers using Gaussian models of class-probability distribution were designed, trained and tested in 3 parts: (1) ability to separate cancer patient samples from controls, (2) cross-validation of classifiers for different cancer types, and (3) correlation of MAC expression with tumor subtype, stage and size. RESULTS: Lung and breast cancer induce MACs in normal buccal mucosa cells. The classifiers based on the selected nuclear features correctly recognized >80% of lung and breast cancer cases. The results indicate that MAC detection is not dependent on the tumor subtype, stage or size. CONCLUSION: The presence of MACs in buccal mucosa cells offers the potential for developing a new noninvasive cancer screening test. |
| | |
Authors:
|
Marija Us-Krasovec; Janez Erzen; Mario Zganec; Margareta Strojan-Flezar; Jaka Lavrencak; David Garner; Alexei Doudkine; Branko Palcic |
Related Documents
:
|
19688127 - Clinical factors related to suspected second primary lung cancer development in patient... 19058197 - Tuberculosis and subsequent risk of lung cancer in xuanwei, china. 18678547 - Quality of care for lung cancer in taiwan: a pattern of care based on core measures in ... 11770217 - Economic analyses and lung cancer. 14706507 - Luteinizing hormone-releasing hormone agonists in the treatment of men with prostate ca... 16926177 - Polymorphisms of stk15 (aurora-a) gene and lung cancer risk in caucasians. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology Volume: 27 ISSN: 0884-6812 ISO Abbreviation: Anal. Quant. Cytol. Histol. Publication Date: 2005 Oct |
Date Detail:
|
Created Date: 2006-02-01 Completed Date: 2006-09-12 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8506819 Medline TA: Anal Quant Cytol Histol Country: United States |
Other Details:
|
Languages: eng Pagination: 254-62 Citation Subset: IM |
Affiliation:
|
Department of Cytopathology, Institute of Oncology, Ljubljana, Slovenia. mus@onko-i.si |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aged, 80 and over Breast Neoplasms / classification, diagnosis*, pathology Case-Control Studies Epithelial Cells / pathology* Female Humans Lung Neoplasms / classification, diagnosis*, pathology Middle Aged Mouth Mucosa / pathology* Neoplasm Staging ROC Curve |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Aneuploidy, centrosome alteration and securin overexpression as features of pituitary somatotroph an...
Next Document: Proteomic analysis of protein expression in prostate cancer.