Document Detail


Malic dehydrogenase activity at 101 C under hydrostatic pressure.
MedLine Citation:
PMID:  14476119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morita, Richard Y. (University of Nebraska, Lincoln) and Roger D. Haight. Malic dehydrogenase activity at 101 C under hydrostatic pressure. J. Bacteriol. 83:1341-1346. 1962.-No malic dehydrogenase activity was found to occur at 101 C at various hydrostatic pressures from 1 to 700 atm. However, activity was demonstrated with hydrostatic pressures above 700 atm, with optimal activity at 1,300 atm at the same temperature. Explanation of the data is based upon thermal denaturation of the enzyme, which involves a molecular volume increase of the enzyme. The molecular volume increase is counteracted by hydrostatic pressure. Pressures above 700 atm to 1,500 atm (highest employed) were sufficient to offset the denaturation by 101 C which probably resulted in an incomplete denaturation of malic dehydrogenase.
Authors:
R Y MORITA; R D HAIGHT
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  83     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1962 Jun 
Date Detail:
Created Date:  1962-12-01     Completed Date:  1998-11-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  1341-6     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Atmospheric Pressure*
Hot Temperature*
Oxidoreductases / chemistry*
Chemical
Reg. No./Substance:
EC 1.-/Oxidoreductases
Comments/Corrections

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