Document Detail


Male:female sex ratio in births resulting from IVF according to swim-up versus Percoll preparation of inseminated sperm.
MedLine Citation:
PMID:  7979811     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two centers have independently reported a higher rate of male to female births following insemination of sperm prepared by a modified swim-up technique. The principle of the modified swim-up is that a small percentage of the x-bearing sperm are the fastest and travel to the top of the supernatant, followed by the y-bearing sperm; the bulk of the x-bearing sperm remain in the pellet. In this technique, the very top layer is discarded and the resulting supernatant is collected, leaving only the pellet. In contrast, with the conventional swim-up technique, the entire supernatant is collected. The study presented herein retrospectively evaluated the male to female sex ratio of births from in vitro fertilization using standard swim-up technique and compared these results to the ratio obtained from separating with Percoll. There were 53% male births with swim-up vs. 54% with Percoll in singleton pregnancies and 51% males with swim-up vs. 40% with Percoll with multiple births. Thus, conventional swim-up alone does not increase percentage of male births.
Authors:
J H Check; D Kwirenk; D Katsoff; M Press; E Breen; A Baker
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of andrology     Volume:  33     ISSN:  0148-5016     ISO Abbreviation:  Arch. Androl.     Publication Date:    1994 Jul-Aug
Date Detail:
Created Date:  1994-12-08     Completed Date:  1994-12-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806755     Medline TA:  Arch Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  63-5     Citation Subset:  IM    
Affiliation:
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / methods
Centrifugation, Zonal / methods
Female
Fertilization in Vitro*
Humans
Infant, Newborn
Male
Povidone
Pregnancy
Pregnancy Outcome
Retrospective Studies
Sex Ratio*
Silicon Dioxide
Spermatozoa / cytology*
Triplets
Twins
Chemical
Reg. No./Substance:
65455-52-9/Percoll; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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