| Male and female rats with severe protein restriction present delayed wound healing. | |
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MedLine Citation:
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PMID: 20029510 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Malnutrition remains a significant problem, not only in developing countries, but also in the developed world. The aim of this study was to investigate the effects of protein restriction on rat excisional cutaneous healing. Male and female rats (12 weeks old) were exposed to different degrees of protein restriction (23%, 12% (slight restriction), and 0% (severe restriction)) for 12 weeks. On week 9, a full-thickness excisional skin lesion was performed, and the lesion area was measured to evaluate wound contraction and re-epithelialization. Euthanasia was performed after 12 weeks, and the lesion and adjacent skin were removed, fixed in formalin, and embedded in paraffin. Sections were stained with hematoxylin-eosin, toluidine blue, picro-mallory, and sirius red, and were immunostained for alpha-smooth muscle actin. Animals (males and females) exposed to severe protein restriction (0% protein) presented impairment of wound contraction. Inflammatory cells were present in higher amounts in the protein-restricted groups than in the 23% group. Extracellular matrix was poorly deposited in the severely restricted group (0%), but only mildly disturbed in the slightly restricted group (12%). Neovascularization was disturbed in both restricted groups. Our study demonstrates that animals exposed to slight protein restriction present disturbed wound healing, but animals exposed to severe protein restriction present impaired wound healing. |
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Authors:
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Marcela Otranto; Isabel Souza-Netto; M?rcia Barbosa Aguila; Andr?a Monte-Alto-Costa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied physiology, nutrition, and metabolism = Physiologie appliqu?e, nutrition et m?tabolisme Volume: 34 ISSN: 1715-5312 ISO Abbreviation: Appl Physiol Nutr Metab Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-04-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101264333 Medline TA: Appl Physiol Nutr Metab Country: Canada |
Other Details:
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Languages: eng Pagination: 1023-31 Citation Subset: IM |
Affiliation:
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Histology and Embryology Departament, State University of Rio de Janeiro, Rio de Janeiro, Brazil. amacosta@uerj.br |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Blood Proteins / analysis Body Weight Diet, Protein-Restricted* Extracellular Matrix / pathology Female Granulation Tissue / blood supply, pathology, physiopathology* Immunohistochemistry Male Mast Cells / pathology, physiology Neovascularization, Physiologic Protein Deficiency / physiopathology* Rats Rats, Wistar Serum Albumin / analysis Sex Characteristics Skin / injuries, physiopathology Wound Healing / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Blood Proteins; 0/Serum Albumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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