Document Detail

Male and female odors induce Fos expression in chemically defined neuronal population.
MedLine Citation:
PMID:  19857504     Owner:  NLM     Status:  MEDLINE    
Olfactory information modulates innate and social behaviors in rodents and other species. Studies have shown that the medial nucleus of the amygdala (MEA) and the ventral premammillary nucleus (PMV) are recruited by conspecific odor stimulation. However, the chemical identity of these neurons is not determined. We exposed sexually inexperienced male rats to female or male odors and assessed Fos immunoreactivity (Fos-ir) in neurons expressing NADPH diaphorase activity (NADPHd, a nitric oxide synthase), neuropeptide urocortin 3, or glutamic acid decarboxylase mRNA (GAD-67, a GABA-synthesizing enzyme) in the MEA and PMV. Male and female odors elicited Fos-ir in the MEA and PMV neurons, but the number of Fos-immunoreactive neurons was higher following female odor exposure, in both nuclei. We found no difference in odor induced Fos-ir in the MEA and PMV comparing fed and fasted animals. In the MEA, NADPHd neurons colocalized Fos-ir only in response to female odors. In addition, urocortin 3 neurons comprise a distinct population and they do not express Fos-ir after conspecific odor stimulation. We found that 80% of neurons activated by male odors coexpressed GAD-67 mRNA. Following female odor, 50% of Fos neurons coexpressed GAD-67 mRNA. The PMV expresses very little GAD-67, and virtually no colocalization with Fos was observed. We found intense NADPHd activity in PMV neurons, some of which coexpressed Fos-ir after exposure to both odors. The majority of the PMV neurons expressing NADPHd colocalized cocaine- and amphetamine-regulated transcript (CART). Our findings suggest that female and male odors engage distinct neuronal populations in the MEA, thereby inducing contextualized behavioral responses according to olfactory cues. In the PMV, NADPHd/CART neurons respond to male and female odors, suggesting a role in neuroendocrine regulation in response to olfactory cues.
Jose Donato; Judney Cley Cavalcante; Renata Juliana Silva; Aline Stavin Teixeira; Jackson Cioni Bittencourt; Carol Fuzeti Elias
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Physiology & behavior     Volume:  99     ISSN:  1873-507X     ISO Abbreviation:  Physiol. Behav.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-17     Completed Date:  2010-03-01     Revised Date:  2013-07-09    
Medline Journal Info:
Nlm Unique ID:  0151504     Medline TA:  Physiol Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-77     Citation Subset:  IM    
Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-900, Brazil.
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MeSH Terms
Amygdala / cytology*
Fasting / physiology
Gene Expression Regulation / physiology
Glutamate Decarboxylase / genetics,  metabolism
Hypothalamus, Posterior / abnormalities,  cytology*
NADPH Dehydrogenase / genetics,  metabolism
Neurons / physiology*
Olfactory Pathways / metabolism
Oncogene Proteins v-fos / metabolism*
RNA, Messenger / metabolism
Sex Characteristics*
Urocortins / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Oncogene Proteins v-fos; 0/RNA, Messenger; 0/Urocortins; EC Dehydrogenase; EC Decarboxylase; EC decarboxylase 1

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