Document Detail

Making immortalized cell lines from embryonic mouse kidney.
MedLine Citation:
PMID:  22639260     Owner:  NLM     Status:  MEDLINE    
Immortalized cell lines derived from embryonic mouse kidneys are useful tools for exploring signaling pathways, morphogenetic mechanisms, and gene function in renal development: they also provide a means for efficient first-round screening of panels of small molecules intended to combat renal pathologies such as the development of cysts, and such cell line-based screening can allow a valuable reduction in the numbers of animals needed for a given line of research. This chapter presents a simple method for generating cell lines from the "Immortomouse," which carries a temperature-sensitive SV40 antigen, under the control of an interferon-regulated promoter.
Guanping Tai; Peter Hohenstein; Jamie A Davies
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  886     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-28     Completed Date:  2012-09-18     Revised Date:  2014-05-13    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-71     Citation Subset:  IM    
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MeSH Terms
Antigens, Polyomavirus Transforming / genetics
Cell Culture Techniques / methods*
Cell Line / cytology*
Clone Cells / cytology
Gene Expression
Kidney / embryology*
Mice, Transgenic
Simian virus 40 / genetics
Grant Support
G0700480/1//National Centre for the Replacement, Refinement and Reduction of Animals in Research
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming

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