Document Detail

Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging in Oocytes.
MedLine Citation:
PMID:  23138948     Owner:  NLM     Status:  In-Data-Review    
Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.
Mark Levasseur
Related Documents :
24361298 - Prestin at year 14: progress and prospect.
23836038 - Label-free quantification proteomics reveals novel calcium binding proteins in matrix v...
11943018 - Enhanced expression of decay-accelerating factor and cd59/homologous restriction factor...
24185898 - Autophagy proteins control goblet cell function by potentiating reactive oxygen species...
6368528 - Electron transfer between plastocyanin and p700 in highly-purified photosystem i reacti...
17986448 - The atg12-atg5 conjugate has a novel e3-like activity for protein lipidation in autophagy.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  121-34     Citation Subset:  IM    
Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle, UK,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Using FRET to Study RanGTP Gradients in Live Mouse Oocytes.
Next Document:  RNAi-Based Methods for Gene Silencing in Mouse Oocytes.