Document Detail


Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging in Oocytes.
MedLine Citation:
PMID:  23138948     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.
Authors:
Mark Levasseur
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  121-34     Citation Subset:  IM    
Affiliation:
Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle, UK, m.d.levasseur@ncl.ac.uk.
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