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Major furocoumarins in grapefruit juice II: Phototoxicity, photogenotoxicity, and inhibitory potency vs. cytochrome P450 3A4 activity.
MedLine Citation:
PMID:  22155270     Owner:  NLM     Status:  Publisher    
Furocoumarins are phototoxic and photogenotoxic natural constituents occurring in a broad variety of plants used in cosmetics, food, and drugs. Grapefruit juice is considered as a major dietary source of furocoumarins although very few is known about the phototoxic properties of furocoumarins in grapefruit. Here we analyzed the phototoxicity and photogenotoxicity of the three major furocoumarins previously found in commercial and freshly prepared grapefruit juices, i.e. bergaptol, bergamottin, and 6',7'-dihydroxy-bergamottin (DHB). We found that DHB was much less toxic than the reference furocoumarin 5-methoxypsoralen (5-MOP) in V79 cells irradiated with UVA light, while bergamottin and bergaptol were not phototoxic. The photomutagenic potency, tested in the V79 HPRT mutagenicity assay, was found to be in the rank order 5-MOP>>DHB>bergamottin while bergaptol was not photomutagenic. In the V79 micronucleus assay, DHB was about 1/7 as potent as 5-MOP whereas bergaptol and bergamottin were inactive. In a cytochrome P450 3A4 inhibition assay in human liver microsomes, IC(50) values of 1.2μM (DHB), 4.5μM (bergamottin), and 77.5μM (bergaptol) were found suggesting that all three furocoumarins contribute to the CYP3A4-inhibitory effect of grapefruit juice. These findings demonstrate that the sum of furocoumarins overestimates the phototoxic properties of grapefruit juice.
Anastasia Messer; Nicole Raquet; Christiane Lohr; Dieter Schrenk
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-3
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  -     ISSN:  1873-6351     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Food Chemistry and Toxicology, University of Kaiserslautern, D-67663 Kaiserslautern, Germany.
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