| Maize meiotic mutants with improper or non-homologous synapsis due to problems in pairing or synaptonemal complex formation. | |
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MedLine Citation:
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PMID: 20926553 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During meiotic prophase homologous chromosomes find each other and pair. Then they synapse, as the linear protein core (axial element or lateral element) of each homologous chromosome is joined together by a transverse central element, forming the tripartite synaptonemal complex (SC). Ten uncloned Zea mays mutants in our collection were surveyed by transmission electron microscopy by making silver-stained spreads of SCs to identify mutants with non-homologous synapsis or improper synapsis. To analyse the mutants further, zyp1, the maize orthologue of the Arabidopsis central element component ZYP1 was cloned and an antibody was made against it. Using antibodies against ZYP1 and the lateral element components AFD1 and ASY1, it was found that most mutants form normal SCs but are defective in pairing. The large number of non-homologous synapsis mutants defective in pairing illustrates that synapsis and pairing can be uncoupled. Of the ten mutants studied, only dsy2 undergoes normal homologous chromosome recognition needed for homologous pairing. The dsy2 mutation fails to maintain the SC. ZYP1 elongation is blocked at zygotene, and only dots of ZYP1 are seen at prophase I. Another mutant, mei*N2415 showed incomplete but homologous synapsis and ASY1 and AFD1 have a normal distribution. Although installation of ZYP1 is initiated at zygotene, its progression is slowed down and not completed by pachytene in some cells and ZYP1 is not retained on pachytene chromosomes. The mutants described here are now available through the Maize Genetics Cooperation Stock Center (http://maizecoop.cropsci.uiuc.edu/). |
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Authors:
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Inna N Golubovskaya; C J Rachel Wang; Ljudmilla Timofejeva; W Zacheus Cande |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-10-06 |
Journal Detail:
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Title: Journal of experimental botany Volume: 62 ISSN: 1460-2431 ISO Abbreviation: J. Exp. Bot. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-01 Completed Date: 2012-05-04 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 9882906 Medline TA: J Exp Bot Country: England |
Other Details:
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Languages: eng Pagination: 1533-44 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cell Biology, University of California-Berkeley, CA 94720, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics Arabidopsis Proteins / genetics, immunology Cell Cycle Proteins / genetics Chromosome Pairing / genetics, physiology* Chromosomes, Plant / metabolism*, ultrastructure Crossing Over, Genetic / physiology* Immunohistochemistry Meiosis / genetics Microscopy, Electron, Transmission Mutation Phenotype Plant Proteins / genetics, immunology, metabolism* Silver Staining Synaptonemal Complex / genetics, metabolism*, ultrastructure Zea mays / cytology, genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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GM 048547/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Cell Cycle Proteins; 0/Plant Proteins; 0/ZYP1a protein, Arabidopsis; 0/ZYP1b protein, Arabidopsis |
| Comments/Corrections | |
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