Document Detail


Maize food allergy: lipid-transfer proteins, endochitinases, and alpha-zein precursor are relevant maize allergens in double-blind placebo-controlled maize-challenge-positive patients.
MedLine Citation:
PMID:  19669736     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Italian patients with maize anaphylaxis have been shown to have IgE toward two major maize allergens: an alpha-amylase inhibitor and a 9-kDa LTP. A complete study on maize food allergens in patients with positive maize double-blind, placebo-controlled food challenge (DBPCFC) is lacking. The objective was to utilize the three maize protein fractions to identify and characterize the most relevant IgE-binding proteins recognized by the sera of Italian and Swiss patients with either a positive maize-DBPCFC or a history of maize-induced anaphylaxis. Osborne's protein fractions of maize were extracted to obtain water-soluble, total zein, and total protein fractions. Protein IgE-binding capacity was investigated by SDS-PAGE immunoblotting using the sera from DBPCFC-positive patients and from patients with maize-induced anaphylaxis. Purified maize LTP was used to inhibit the IgE immunoblotting of the three protein fractions. IgE immunoblotting demonstrated that the 9-kDa LTP was recognized by all the Italian patients and by none of the Swiss patients. Other allergens were: 14-kDa alpha-amylase inhibitor, 30-kDa endochitinases A and -B, 19 kDa zein-beta precursor, and 26 kDa zein-alpha precursor; a newly described allergen, the globulin-2 precursor, identified in the total protein fraction. It is noteworthy that maize LTP and endochitinase were cross-reactive with grape LTP and one grape endochitinase. LTP was found to be the only major allergen in Italian patients with either positive maize challenge or a history of maize-induced anaphylaxis. We have identified other maize allergens in subjects with maize food allergy, as grape cross-reactive endochitinase, however, the clinical significance of these proteins needs to be investigated in larger groups of patients with allergy to these food items.
Authors:
Elide A Pastorello; Laura Farioli; Valerio Pravettoni; Joseph Scibilia; Amedeo Conti; Donatella Fortunato; Linda Borgonovo; Simona Bonomi; Laura Primavesi; Barbara Ballmer-Weber
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Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't     Date:  2009-08-11
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  395     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  93-102     Citation Subset:  IM    
Affiliation:
Unit of Allergology and Immunology-Niguarda Ca' Granda Hospital, Piazza Ospedale Maggiore 3, 20162 Milan, Italy. allergologia@ospedaleniguarda.it
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Anaphylaxis / etiology
Antigens, Plant
Carrier Proteins / immunology*
Child
Chitinase / immunology*
Double-Blind Method
Female
Food Hypersensitivity / etiology*,  immunology
Humans
Immunoglobulin E / metabolism
Italy
Male
Middle Aged
Placebos
Plant Proteins
Protein Binding
Serologic Tests / methods*
Young Adult
Zea mays / immunology*
Zein / immunology*
Chemical
Reg. No./Substance:
0/Antigens, Plant; 0/Carrier Proteins; 0/Placebos; 0/Plant Proteins; 0/lipid transfer protein; 37341-29-0/Immunoglobulin E; 9010-66-6/Zein; EC 3.2.1.14/Chitinase

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