| Maize food allergy: lipid-transfer proteins, endochitinases, and alpha-zein precursor are relevant maize allergens in double-blind placebo-controlled maize-challenge-positive patients. | |
| | |
MedLine Citation:
|
PMID: 19669736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Italian patients with maize anaphylaxis have been shown to have IgE toward two major maize allergens: an alpha-amylase inhibitor and a 9-kDa LTP. A complete study on maize food allergens in patients with positive maize double-blind, placebo-controlled food challenge (DBPCFC) is lacking. The objective was to utilize the three maize protein fractions to identify and characterize the most relevant IgE-binding proteins recognized by the sera of Italian and Swiss patients with either a positive maize-DBPCFC or a history of maize-induced anaphylaxis. Osborne's protein fractions of maize were extracted to obtain water-soluble, total zein, and total protein fractions. Protein IgE-binding capacity was investigated by SDS-PAGE immunoblotting using the sera from DBPCFC-positive patients and from patients with maize-induced anaphylaxis. Purified maize LTP was used to inhibit the IgE immunoblotting of the three protein fractions. IgE immunoblotting demonstrated that the 9-kDa LTP was recognized by all the Italian patients and by none of the Swiss patients. Other allergens were: 14-kDa alpha-amylase inhibitor, 30-kDa endochitinases A and -B, 19 kDa zein-beta precursor, and 26 kDa zein-alpha precursor; a newly described allergen, the globulin-2 precursor, identified in the total protein fraction. It is noteworthy that maize LTP and endochitinase were cross-reactive with grape LTP and one grape endochitinase. LTP was found to be the only major allergen in Italian patients with either positive maize challenge or a history of maize-induced anaphylaxis. We have identified other maize allergens in subjects with maize food allergy, as grape cross-reactive endochitinase, however, the clinical significance of these proteins needs to be investigated in larger groups of patients with allergy to these food items. |
| | |
Authors:
|
Elide A Pastorello; Laura Farioli; Valerio Pravettoni; Joseph Scibilia; Amedeo Conti; Donatella Fortunato; Linda Borgonovo; Simona Bonomi; Laura Primavesi; Barbara Ballmer-Weber |
Related Documents
:
|
19877566 - Gluten-free food as source of hidden allergen (lupine). 9389296 - Concentrations of the major birch tree allergen bet v 1 in pollen and respirable fine p... 16805416 - Peach fuzz contains large amounts of lipid transfer protein: is this the cause of the h... 17334816 - Life tables of the predatory mite phytoseiulus longipes feeding on tetranychus evansi a... 11580036 - Indirect evidence against delayed "skip-oviposition" behavior by aedes aegypti (diptera... 12676626 - Evaluation of mercury in urine as an indicator of exposure to low levels of mercury vapor. |
Publication Detail:
|
Type: Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't Date: 2009-08-11 |
Journal Detail:
|
Title: Analytical and bioanalytical chemistry Volume: 395 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2009 Sep |
Date Detail:
|
Created Date: 2009-08-11 Completed Date: 2009-10-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 93-102 Citation Subset: IM |
Affiliation:
|
Unit of Allergology and Immunology-Niguarda Ca' Granda Hospital, Piazza Ospedale Maggiore 3, 20162 Milan, Italy. allergologia@ospedaleniguarda.it |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Anaphylaxis / etiology Antigens, Plant Carrier Proteins / immunology* Child Chitinase / immunology* Double-Blind Method Female Food Hypersensitivity / etiology*, immunology Humans Immunoglobulin E / metabolism Italy Male Middle Aged Placebos Plant Proteins Protein Binding Serologic Tests / methods* Young Adult Zea mays / immunology* Zein / immunology* |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Plant; 0/Carrier Proteins; 0/Placebos; 0/Plant Proteins; 0/lipid transfer protein; 37341-29-0/Immunoglobulin E; 9010-66-6/Zein; EC 3.2.1.14/Chitinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A new SIMS paradigm for 2D and 3D molecular imaging of bio-systems.
Next Document: CYP2C9 genotype and pharmacodynamic responses to losartan in patients with primary and secondary kid...