Document Detail

Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB.
MedLine Citation:
PMID:  6319008     Owner:  NLM     Status:  MEDLINE    
Certain derivatives of copy-control mutants of plasmid Clo DF13 are not stably inherited in E. coli. These plasmids, predominantly present as multimeric DNA molecules, lack a specific region, designated parB. Here we present the nucleotide sequence of this parB region spanning 328 bp between 46% and 49% on the plasmid genome. parB is a noncoding region with extensive internal symmetry. A recA-independent, site-specific resolution process occurs between two intramolecular parB sites present in direct orientation relative to each other. A gene located in the direct vicinity of parB, gene L, is not essential for parB functioning. However, our genetic data indicate that transcription from the gene L-containing operon into parB is required. We conclude that the efficient maintenance of Clo DF13 cop derivatives containing parB is provided by resolution of mutimeric molecules. Because Clo DF13 wt and cop derivatives have a different response to the deletion of parB we postulate that two different recombination systems, a parB-dependent and a parB-independent system, operate in the efficient maintenance of Clo DF13 plasmids.
M J Hakkaart; P J van den Elzen; E Veltkamp; H J Nijkamp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  36     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-03-02     Completed Date:  1984-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  203-9     Citation Subset:  IM    
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MeSH Terms
Bacterial Proteins / genetics*
Base Sequence
DNA Transposable Elements
DNA, Bacterial / genetics
Escherichia coli / genetics*
Escherichia coli Proteins*
Genes, Bacterial*
Recombination, Genetic*
Transcription, Genetic
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacteriocins; 0/DNA Transposable Elements; 0/DNA, Bacterial; 0/Escherichia coli Proteins; 0/cloacin DF13 protein, E coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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