Document Detail

Maintenance of Sensitivity of the T-SPOT.TB Assay after Overnight Storage of Blood Samples, Dar es Salaam, Tanzania.
MedLine Citation:
PMID:  22567274     Owner:  NLM     Status:  In-Data-Review    
Background. T-SPOT.TB is an interferon gamma release assay for detecting Mycobacterium tuberculosis infection. The requirement to process within 8 hours is constraining, deters use, and leads to invalid results. Addition of T Cell Xtend reagent may allow delayed processing, but has not been extensively field tested. Design. Consecutive AFB smear positive adult tuberculosis patients were prospectively recruited in Dar es Salaam, Tanzania. Patients provided a medical history, 1-3 sputum samples for culture and 1 blood sample which was transported to the laboratory under temperature-controlled conditions. After overnight storage, 25 μL of T Cell Xtend reagent was added per mL of blood, and the sample was tested using T-SPOT.TB. Results. 143 patients were enrolled: 57 patients were excluded because temperature control was not maintained, 19 patients were excluded due to red blood cell contamination, and one did not provide a sputum sample for culture. Among 66 evaluable patients, overall agreement between T-SPOT.TB and culture was 95.4% (95%CI; 87.1-99.0%) with Kappa value 0.548. Sensitivity of T-SPOT.TB when using T Cell Xtend reagent was 96.8% (95%CI; 88.8-99.6%). Conclusions. When T Cell Xtend reagent is added to specimens held overnight at recommended temperatures, T-SPOT.TB is as sensitive as the standard assay in patients with tuberculosis.
Elizabeth A Talbot; Isaac Maro; Katherine Ferguson; Lisa V Adams; Lillian Mtei; Mecky Matee; C Fordham von Reyn
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Publication Detail:
Type:  Journal Article     Date:  2012-02-06
Journal Detail:
Title:  Tuberculosis research and treatment     Volume:  2012     ISSN:  2090-1518     ISO Abbreviation:  Tuberc Res Treat     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101576351     Medline TA:  Tuberc Res Treat     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  345290     Citation Subset:  -    
Dartmouth Medical School, Hanover, NH 03755, USA.
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