Document Detail

Maintaining high anaerobic succinic acid productivity by product removal.
MedLine Citation:
PMID:  19921276     Owner:  NLM     Status:  MEDLINE    
During dual-phase fermentations using Escherichia coli engineered for succinic acid production, the productivity and viable cell concentration decrease as the concentration of succinic acid increases. The effects of succinic acid on the fermentation kinetics, yield, and cell viability were investigated by resuspending cells in fresh media after selected fermentation times. The cellular succinic acid productivity could be restored, but cell viability continuously decreased throughout the fermentations by up to 80% and subsequently the volumetric productivity was reduced. Omitting complex nutrients in the resuspension media had no significant effect on cellular succinate productivity and yield, although the viable cell concentration and thus the volumetric productivity was reduced by approximately 20%. By resuspending the cells, the amount of succinate produced during a 100-h fermentation was increased by more than 60%. The results demonstrate that by product removal succinic acid productivity can be maintained at high levels for extended periods of time.
Christian Andersson; Ekaterina Petrova; Kris Berglund; Ulrika Rova
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-17
Journal Detail:
Title:  Bioprocess and biosystems engineering     Volume:  33     ISSN:  1615-7605     ISO Abbreviation:  Bioprocess Biosyst Eng     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-13     Completed Date:  2010-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088505     Medline TA:  Bioprocess Biosyst Eng     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  711-8     Citation Subset:  IM    
Division of Chemical Engineering, Luleå University of Technology, 971 87, Luleå, Sweden.
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MeSH Terms
Bacteriological Techniques
Culture Media*
Escherichia coli / enzymology*,  growth & development
Succinic Acid / isolation & purification,  metabolism*,  pharmacology
Time Factors
Reg. No./Substance:
0/Culture Media; 110-15-6/Succinic Acid

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