Document Detail

Magnolol stimulates steroidogenesis in rat adrenal cells.
MedLine Citation:
PMID:  11082125     Owner:  NLM     Status:  MEDLINE    
1. This study investigated the effect of magnolol, a compound purified from Magnolia officinalis, on glucocorticoid production by primary adrenal cell culture. 2. Magnolol increased corticosterone secretion in a dose-dependent manner, this effect being maximal at 40 microM. A similar effect was seen in a minced adrenal gland system. 3. In magnolol-treated cells, the number and total area of cytoplasmic lipid droplets were reduced, suggesting a high utilization rate of cholesterol esters stored in lipid droplets. In control cells, the capsule of the lipid droplet was clearly delineated by immunostaining with antibody A2, whereas capsular staining was discontinuous or undetectable following magnolol treatment. The percentage of decapsulated cells increased significantly from 20% in the control group to 80% in the magnolol-treated group. 4. Magnolol-induced steroidogenesis was not mediated either via the traditional ACTH-cyclic AMP-protein kinase A pathway or by protein kinase C, since the intracellular cyclic AMP level did not change and inhibition of protein kinase A or C did not block the action of magnolol. Furthermore, calcium/calmodulin-dependent protein kinase II was not involved in magnolol-induced steroidogenesis. 5. The stimulatory effect of magnolol on steroidogenesis apparently requires new protein synthesis, since cycloheximide inhibited magnolol-induced corticosterone production by 50%. 6. Although other studies have shown that high concentrations of magnolol inhibit acyl-CoA: cholesterol acyltransferase and 11 beta-hydroxysteroid dehydrogenase in a cell-free system, our data show that, in adrenal cell cultures, low concentrations of magnolol have a stimulatory effect on steroidogenesis, and the glucocorticoid produced may explain the effective control of asthma by Magnolia officinalis.
S M Wang; L J Lee; Y T Huang; J J Chen; Y L Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  131     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-02-02     Completed Date:  2001-02-02     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1172-8     Citation Subset:  IM    
Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan 100, Republic of China.
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MeSH Terms
Adrenal Glands / cytology,  drug effects*,  secretion
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Biphenyl Compounds / pharmacology*
Cells, Cultured
Corticosterone / biosynthesis*,  secretion
Cycloheximide / pharmacology
Dose-Response Relationship, Drug
Lipid Metabolism
Protein Synthesis Inhibitors / pharmacology
Rats, Wistar
Signal Transduction / drug effects,  physiology
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Biphenyl Compounds; 0/Lignans; 0/Protein Synthesis Inhibitors; 001E35HGVF/magnolol; 50-22-6/Corticosterone; 66-81-9/Cycloheximide

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