Document Detail

Magnetic targeting of iron-oxide-labeled fluorescent hepatoma cells to the liver.
MedLine Citation:
PMID:  19137308     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to determine whether an external magnet field can induce preferential trafficking of magnetically labeled Huh7 hepatoma cells to the liver following liver cell transplantation. Huh7 hepatoma cells were labeled with anionic magnetic nanoparticles (AMNP) and tagged with a fluorescent membrane marker (PKH67). Iron-uptake was measured by magnetophoresis. Twenty C57Bl6 mice received an intrasplenic injection of 2 x 10(6) labeled cells. An external magnet (0.29 T; 25 T/m) was placed over the liver of 13 randomly selected animals (magnet group), while the remaining 7 animals served as controls. MRI (1.5 T) and confocal fluorescence microscopy (CFM) were performed 10 days post-transplantation. The presence and location of labeled cells within the livers were compared in the magnet group and controls, and confronted with histological analysis representing the standard of reference. Mean iron content per cell was 6 pg. Based on histology, labeled cells were more frequently present within recipient livers in the magnet group (p < 0.01) where their distribution was preferentially peri-vascular (p < 0.05). MRI and CFM gave similar results for the overall detection of transplanted cells (kappa = 0.828) and for the identification of peri-vascular cells (kappa = 0.78). Application of an external magnet can modify the trafficking of transplanted cells, especially by promoting the formation of perivascular aggregates.
Alain Luciani; Claire Wilhelm; Patrick Bruneval; Patrick Cunin; Gwennhael Autret; Alain Rahmouni; Olivier Clément; Florence Gazeau
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Publication Detail:
Type:  Journal Article     Date:  2009-01-10
Journal Detail:
Title:  European radiology     Volume:  19     ISSN:  1432-1084     ISO Abbreviation:  Eur Radiol     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-01     Completed Date:  2009-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9114774     Medline TA:  Eur Radiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1087-96     Citation Subset:  IM    
Laboratoire de Recherche en Imagerie, EA 4062, Université René Descartes, Hôpital Européen Georges Pompidou, Assistance Publique - Hôpitaux de Paris, 20 rue Leblanc, 75015 Paris, France.
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MeSH Terms
Carcinoma, Hepatocellular / diagnosis*
Cell Line, Tumor
Cell Transplantation / methods
Ferric Compounds / diagnostic use*
Fluorescent Dyes / diagnostic use*
Liver / pathology
Liver Neoplasms / diagnosis*
Magnetic Resonance Imaging / methods
Mice, Inbred C57BL
Microscopy, Confocal / methods
Microscopy, Electron, Transmission / methods
Spleen / pathology
Reg. No./Substance:
0/Ferric Compounds; 0/Fluorescent Dyes; 1309-37-1/ferric oxide

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