Document Detail


Magnetic stimulation of one-dimensional neuronal cultures.
MedLine Citation:
PMID:  18326634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcranial magnetic stimulation is a remarkable tool for neuroscience research, with a multitude of diagnostic and therapeutic applications. Surprisingly, application of the same magnetic stimulation directly to neurons that are dissected from the brain and grown in vitro was not reported to activate them to date. Here we report that central nervous system neurons patterned on large enough one-dimensional rings can be magnetically stimulated in vitro. In contrast, two-dimensional cultures with comparable size do not respond to excitation. This happens because the one-dimensional pattern enforces an ordering of the axons along the ring, which is designed to follow the lines of the magnetically induced electric field. A small group of sensitive (i.e., initiating) neurons respond even when the network is disconnected, and are presumed to excite the entire network when it is connected. This implies that morphological and electrophysiological properties of single neurons are crucial for magnetic stimulation. We conjecture that the existence of a select group of neurons with higher sensitivity may occur in the brain in vivo as well, with consequences for transcranial magnetic stimulation.
Authors:
Assaf Rotem; Elisha Moses
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-07
Journal Detail:
Title:  Biophysical journal     Volume:  94     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-29     Completed Date:  2008-06-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5065-78     Citation Subset:  IM    
Affiliation:
Weizmann Institute of Science, Physics of Complex Systems, Rehovot, Israel. assaf.rotem@weizmann.ac.il
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MeSH Terms
Descriptor/Qualifier:
Action Potentials / physiology*
Animals
Cells, Cultured
Computer Simulation
Electric Stimulation / methods*
Humans
Magnetics*
Models, Neurological*
Nerve Net / physiology*
Neurons / physiology*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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