Document Detail


Magnetic resonance microscopy of the barramundi (Lates calcarifer) brain.
MedLine Citation:
PMID:  20967831     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Magnetic resonance imaging (MRI) is an established technique for morphological imaging of the central nervous system. Despite its prevalent use in a range of taxa, few studies exist on the brains of teleosts. In this study, we present a detailed analysis of a teleost brain using high-resolution MRI. Images were acquired from a Bruker 16.4 T vertical magnet with a three-dimensional flash T(2)*-weighted image sequence. High contrast was obtained using Magnevist® and the following imaging parameters: a flip angle of 30°, a repetition time of 50 ms, an echo time of 14 ms, and an image matrix of 1024 × 400 × 400. The resulting isotropic resolution of 30 μm allowed us to thoroughly describe the architecture of the barramundi (Lates calcarifer) brain, including descriptions of nuclei, fiber tracts, and cellular layers. A good correspondence, both in contrast and morphology, was found between magnetic resonance images and Nissl-stained brain sections, allowing for an analysis of the benefits and drawbacks of MRI and conventional histology.
Authors:
Jeremy F P Ullmann; Gary Cowin; Shaun P Collin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of morphology     Volume:  271     ISSN:  1097-4687     ISO Abbreviation:  J. Morphol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0406125     Medline TA:  J Morphol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1446-56     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc..
Affiliation:
Sensory Neurobiology Group, School of Biomedical Sciences, The University of Queensland, Brisbane 4072, Queensland, Australia. j.ullmann@uq.edu.au
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