| Magnetic resonance imaging and immunoblot analyses in rats with experimentally induced cerebral alveolar echinococcosis. | |
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MedLine Citation:
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PMID: 14727814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The early stage of experimentally induced secondary cerebral alveolar echinococcosis (AE) in rats was investigated by use of magnetic resonance imaging (MRI) and immunoblot (western blot) analyses. Thirty-six female Wistar rats (6 to 8 weeks old) were injected intracranially with a 10% homogenate of echinococcal larval tissues in which the concentrations of microvesicles and protoscolices were estimated to be 3.8 and 1.5 x 10(4)/ml, respectively. To observe the fine structure of the rat brain, MRI was performed under a high magnetic field of 7.05 T. Histologic examination also was performed. The T2-weighted MR images revealed a hyperintense region in the cerebral cortex at two weeks after injection of the homogenate. At three weeks after injection, this region was found to have cysts on the basis of results of histologic examination. Signal-void regions corresponding to hyperplasia and the subsequent calcification of the cuticle layer at six and 13 weeks after injection, respectively, were observed in T2-weighted and proton density MR images. On the other hand, at nine weeks after injection, AE was discernible by use of western blot analysis of sera with antibodies of two epitopes (Em18 and Em16) of E. multilocularis. Using this secondary cerebral AE animal model, it was concluded that the MRI method was suitable for early detection of secondary cerebral AE. |
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Authors:
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Taketoshi Asanuma; Yukari Matsumoto; Mitsuyoshi Takiguchi; Osamu Inanami; Minoru Nakao; Kazuhiro Nakaya; Akira Ito; Akira Hashimoto; Mikinori Kuwabara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Comparative medicine Volume: 53 ISSN: 1532-0820 ISO Abbreviation: Comp. Med. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2004-01-19 Completed Date: 2004-04-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100900466 Medline TA: Comp Med Country: United States |
Other Details:
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Languages: eng Pagination: 649-56 Citation Subset: IM |
Affiliation:
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Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Helminth / blood Blotting, Western / methods* Brain Diseases / diagnosis*, immunology, parasitology Disease Models, Animal* Echinococcosis / diagnosis*, etiology, immunology Echinococcus / immunology, isolation & purification, pathogenicity Female Helminthiasis, Animal / diagnosis, immunology Magnetic Resonance Imaging* Rats Rats, Wistar Rodent Diseases / diagnosis*, parasitology Specific Pathogen-Free Organisms |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Helminth |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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