Document Detail

Magnetic resonance imaging of human embryonic stem cells.
MedLine Citation:
PMID:  19653198     Owner:  NLM     Status:  MEDLINE    
Magnetic resonance imaging (MRI) may emerge as an ideal non-invasive imaging modality to monitor stem cell therapy in the failing heart. This imaging modality generates any arbitrary tomographic view at high spatial and temporal resolution with exquisite intrinsic tissue contrast. This capability enables robust evaluation of both the cardiac anatomy and function. Traditionally, superparamagnetic iron oxide nanoparticle (SPIO) has been widely used for cellular MRI due to SPIO's ability to enhance sensitivity of MRI by inducing remarkable hypointense, negative signal, "blooming effect" on T2*-weighted MRI acquisition. Recently, manganese chloride (MnCl(2)) has been reported by our laboratory for its ability as a contrast agent to track biological activity of viable cells. Hyperintense, positive signals can be achieved from the Mn(2+)-labeled stem cells on T1-weighted MRI acquisition. Cytotoxicity is a potential drawback of Mn(2+) labeling of the cells. However, in our laboratory the labeling method has been optimized to minimize cytotoxic effects. This article describes two different magnetic labeling methods of human embryonic stem cells (hESC) using SPIO and MnCl(2).
Jaehoon Chung; Mayumi Yamada; Phillip C Yang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in stem cell biology     Volume:  Chapter 5     ISSN:  1938-8969     ISO Abbreviation:  Curr Protoc Stem Cell Biol     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-04     Completed Date:  2009-10-01     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  101470226     Medline TA:  Curr Protoc Stem Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 5A.3     Citation Subset:  IM    
Stanford University School of Medicine, Stanford, California, USA.
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MeSH Terms
Cell Culture Techniques
Cells, Cultured
Chlorides / chemistry
Coated Materials, Biocompatible / chemistry
Collagen / chemistry
Contrast Media / chemistry
Drug Combinations
Embryonic Stem Cells / chemistry,  cytology*,  metabolism,  transplantation
Ferumoxytol / chemistry
Laminin / chemistry
Magnetic Resonance Imaging / methods*
Magnetite Nanoparticles
Manganese Compounds / chemistry
Metal Nanoparticles / chemistry
Myocardium / cytology
Protamines / metabolism
Proteoglycans / chemistry
Sensitivity and Specificity
Staining and Labeling / methods
Stem Cell Transplantation / methods
Time Factors
Transplantation, Heterologous / methods
Reg. No./Substance:
0/Chlorides; 0/Coated Materials, Biocompatible; 0/Contrast Media; 0/Drug Combinations; 0/Laminin; 0/Magnetite Nanoparticles; 0/Manganese Compounds; 0/Protamines; 0/Proteoglycans; 119978-18-6/matrigel; 1317-61-9/Ferumoxytol; 9004-54-0/Dextrans; 9007-34-5/Collagen; G6N3J05W84/ferumoxides; QQE170PANO/manganese chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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