| Magnetic resonance imaging of cardiomyocyte apoptosis with a novel magneto-optical nanoparticle. | |
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MedLine Citation:
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PMID: 16086367 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability to image cardiomyocyte apoptosis in vivo with high-resolution MRI could facilitate the development of novel cardioprotective therapies. The sensitivity of the novel nanoparticle AnxCLIO-Cy5.5 for cardiomyocyte apoptosis was thus compared in vitro to that of annexin V-FITC and showed a high degree of colocalization. MRI was then performed, following transient coronary artery (LAD) occlusion, in five mice given AnxCLIO-Cy5.5 and in four mice given an identical dose (2 mg Fe/kg) of CLIO-Cy5.5. MR signal intensity and myocardial T2* were evaluated, in vivo, in hypokinetic regions of myocardium in the LAD distribution. Ex vivo fluorescence imaging was performed to confirm the in vivo findings. Myocardial T2* was significantly lower in the mice given AnxCLIO-Cy5.5 (8.1 versus 13.2 ms, P<0.01), and fluorescence target to background ratio was significantly higher (2.1 versus 1.1, P<0.01). This study thus demonstrates the feasibility of obtaining high-resolution MR images of cardiomyocyte apoptosis in vivo with the novel nanoparticle, AnxCLIO-Cy5.5. |
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Authors:
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David E Sosnovik; Eyk A Schellenberger; Matthias Nahrendorf; Mikhail S Novikov; Takashi Matsui; George Dai; Fred Reynolds; Luanda Grazette; Anthony Rosenzweig; Ralph Weissleder; Lee Josephson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 54 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-08-29 Completed Date: 2005-12-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 718-24 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2005 Wiley-Liss, Inc. |
Affiliation:
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Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA. Sosnovik@nmr.mgh.harvard.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Annexin A5 / pharmacokinetics* Apoptosis* Contrast Media / pharmacokinetics* Magnetic Resonance Imaging / methods* Mice Mice, Inbred C57BL Microscopy, Fluorescence Myocytes, Cardiac Nanotechnology |
| Grant Support | |
ID/Acronym/Agency:
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CA91807/CA/NCI NIH HHS; P50CA86355/CA/NCI NIH HHS; R01CA86782/CA/NCI NIH HHS; R24 CA92782/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Contrast Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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