Document Detail

Magnetic particles functionalized with PAMAM-dendrimers and antibodies: a new system for an ELISA method able to detect Ara h3/4 peanut allergen in foods.
MedLine Citation:
PMID:  20607526     Owner:  NLM     Status:  MEDLINE    
An innovative enzyme-linked immunosorbent assay (ELISA) format based on antibody-coated magnetic micro-particles (MPs) for the sensitive detection of Ara h3/4 allergen in food is described. The immunosupport is suspended in the incubation solutions and the MPs with the captured allergen can be easily harvested on a magnet, separated from the solutions, and washed using an easy-to-use, fast and selective approach that allows its detection and quantification. Two differently coated MPs, ProteinA-Pn-b and MP-NH(2)-PAMAM G 1.5-Pn-b immunosupports, were tested. The functionalization of the MPs with PAMAM-sodium carboxylate dendrimers elicits a major stability on the immunoglobulin activity resulting in a threefold enhancement of the analytical sensitivity for the assay with respect to a ProteinA immobilization. Validation was carried out on two different matrices: corn flakes and biscuits. In the case of MP-NH(2)-PAMAM G 1.5 -Pn-b immunosupport, limit of detection was found to be 0.2 mg peanuts/kg matrix in both matrices; the linear response range was demonstrated from 2.5 to 15 mg peanuts/kg matrix by performing statistical tests (homoscedasticity and Mandel fitting tests). Good accuracy and recovery (>80 +/- 2%) were obtained. Different food samples were tested and the results were compared with those obtained with a commercially available ELISA kit. The results obtained in this work demonstrated the applicability of the immunomagnetic ELISA methods on real samples and the possibility to perform the assay with significantly reduced reagent and sample consumption.
Francesca Speroni; Lisa Elviri; Maria Careri; Alessandro Mangia
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-10
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  397     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3035-42     Citation Subset:  IM    
Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Viale G.P. Usberti 17/A, 43100 Parma, Italy.
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MeSH Terms
Allergens / analysis*,  immunology
Antibodies / analysis,  immunology
Antigens, Plant / analysis*,  immunology
Arachis hypogaea / chemistry,  immunology
Dendrimers / chemistry
Enzyme-Linked Immunosorbent Assay / instrumentation,  methods*
Food Analysis*
Glycoproteins / analysis*,  immunology
Plant Proteins / analysis*,  immunology
Seed Storage Proteins / analysis*,  immunology
Reg. No./Substance:
0/Allergens; 0/Antibodies; 0/Antigens, Plant; 0/Ara h 4 allergen, Arachis hypogaea; 0/Dendrimers; 0/Glycoproteins; 0/PAMAM Starburst; 0/Plant Proteins; 0/Seed Storage Proteins; 0/allergen Ara h3

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