| Magnetic loading of tyrosinase-Fe(3)O(4)/mesoporous silica core/shell microspheres for high sensitive electrochemical biosensing. | |
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MedLine Citation:
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PMID: 21237311 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A new protocol is proposed for magnetic loading and sensitive electrochemical detection of phenol via the tyrosinase cross-linked mesoporous magnetic core/shell microspheres. The mesoporous magnetic microspheres, characterized by transmission electron microscopy, N(2) adsorption/desorption isotherms, and magnetic curve displays high capacity for enzyme immobilization and strong magnetism to adhere to the magnetic electrode surface without any additional adhesive reagent. The biosensor exhibits a wide linear response to phenol ranging from 1.0×10(-9) to 1.0×10(-5)M, a high sensitivity of 78μAmM(-1), a low detection limit of 1nM, and a fast response rate (less than 5s). The proposed method is simple, rapid, inexpensive and convenient in electrode renewal, which is recommended as a promising experimental platform for wider applications in biosensing. |
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Authors:
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Shuo Wu; Hainan Wang; Shengyang Tao; Chan Wang; Lihui Zhang; Zhiguang Liu; Changgong Meng |
Publication Detail:
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Type: Journal Article Date: 2010-12-08 |
Journal Detail:
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Title: Analytica chimica acta Volume: 686 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 81-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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School of Chemistry, Dalian University of Technology, Dalian 116023, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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