| Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells. | |
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MedLine Citation:
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PMID: 19946957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4(+) CD25(+) regulatory T (Treg) cells, in order to inform further studies in Treg cell function. METHODS: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4(+) CD25(+) Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4(+) CD25(+) Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4(+) CD25(+) Treg cells in the in vitro proliferation experiments. RESULTS: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4(+) CD25(+) Treg cells, however, obtained with both methods have similar immunosuppressive capacities. CONCLUSION: The result suggests that both methods can be used in isolating CD4(+) CD25(+) Treg cells, and one can select the best method according to specific needs and availability of the methodologies. |
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Authors:
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Hang Yan; Chen-guang Ding; Pu-xun Tian; Guan-qun Ge; Zhan-kui Jin; Li-ning Jia; Xiao-ming Ding; Xiao-ming Pan; Wu-jun Xue |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Zhejiang University. Science. B Volume: 10 ISSN: 1862-1783 ISO Abbreviation: J Zhejiang Univ Sci B Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-30 Completed Date: 2010-03-01 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 101236535 Medline TA: J Zhejiang Univ Sci B Country: China |
Other Details:
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Languages: eng Pagination: 928-32 Citation Subset: IM |
Affiliation:
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Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medicine College, Xi'an Jiaotong University, Xi'an 710061, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CD4-Positive T-Lymphocytes / cytology* Cell Separation Cell Survival Flow Cytometry / methods* Immune System Immunosuppressive Agents / pharmacology Interleukin-2 Receptor alpha Subunit / biosynthesis* Lymphocytes / cytology Magnetics Mice Mice, Inbred BALB C |
| Chemical | |
Reg. No./Substance:
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0/Immunosuppressive Agents; 0/Interleukin-2 Receptor alpha Subunit |
| Comments/Corrections | |
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