Document Detail


Magnetic activated cell sorting allows isolation of spermatogonia from adult primate testes and reveals distinct GFRa1-positive subpopulations in men.
MedLine Citation:
PMID:  20015158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Isolation of spermatogonial stem cells (SSCs) could enable in vitro approaches for exploration of spermatogonial physiology and therapeutic approaches for fertility preservation. SSC isolation from adult testes is difficult due to low cell numbers and lacking cell surface markers. Glial cell-derived neurotrophic factor family receptor alpha-1 (GFRalpha1) plays a crucial role for the maintenance of SSCs in rodents and is expressed in monkey spermatogonia. METHODS: Magnetic activated cell sorting was employed for the enrichment of GFRalpha1+ spermatogonia from adult primate testes. RESULTS: Magnetic activated cell sorting of monkey cells enriched GFRalpha1+ cells threefold. 11.4% of GFRalpha1+ cells were recovered. 42.9% of GFRalpha1+ cells were recovered in sorted fractions of human testicular cells, representing a fivefold enrichment. Interestingly, a high degree of morphological heterogeneity among the GFRalpha1+ cells from human testes was observed. CONCLUSIONS: Magnetic activated cell sorting using anti-GFRalpha1 antibodies provides an enrichment strategy for spermatogonia from monkey and human testes.
Authors:
Kathrin Gassei; Jens Ehmcke; Rajiv Dhir; Stefan Schlatt
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-12-15
Journal Detail:
Title:  Journal of medical primatology     Volume:  39     ISSN:  1600-0684     ISO Abbreviation:  J. Med. Primatol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0320626     Medline TA:  J Med Primatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  83-91     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Animals
Cell Separation / methods
Glial Cell Line-Derived Neurotrophic Factor Receptors / biosynthesis*
Histocytochemistry / veterinary
Humans
Macaca mulatta / physiology*
Magnetics
Male
Middle Aged
Spermatogenesis / physiology
Spermatogonia / cytology*,  metabolism
Testis / cytology*,  metabolism
Young Adult
Grant Support
ID/Acronym/Agency:
U54 HD 008610/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Glial Cell Line-Derived Neurotrophic Factor Receptors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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