Document Detail


Magnetic resonance studies of macromolecular content in engineered cartilage treated with pulsed low-intensity ultrasound.
MedLine Citation:
PMID:  20807015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Noninvasive monitoring of matrix development in tissue-engineered cartilage constructs would permit ongoing assessment with the ability to modify culture conditions during development to optimize tissue characteristics. In this study, chondrocytes seeded in a collagen hydrogel were exposed for 20 min/day to pulsed low-intensity ultrasound (PLIUS) at 30 mWcm(-2) and cultured for up to 5 weeks. Biochemical assays, histology, immunohistochemistry, Fourier transform infrared spectroscopy, and magnetic resonance imaging (MRI) were performed at weeks 3 and 5 after initiation of growth. The noninvasive MRI measurements were correlated with those from the invasive studies. In particular, MRI transverse relaxation time (T2) and magnetization transfer rate (k(m)) correlated with macromolecular content, which was increased by application of PLIUS. This indicates the sensitivity of MR techniques to PLIUS-induced changes in matrix development, and highlights the potential for noninvasive assessment of the efficacy of anabolic interventions for engineered tissue.
Authors:
Onyi N Irrechukwu; Ping-Chang Lin; Kate Fritton; Steve Doty; Nancy Pleshko; Richard G Spencer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2010-10-25
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  17     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-31     Completed Date:  2011-05-02     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  407-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage, Articular / cytology,  physiology*,  radiation effects
Cattle
Cells, Cultured
Chondrocytes / cytology,  physiology*,  radiation effects
Chondrogenesis / physiology*,  radiation effects
Glycosaminoglycans / metabolism*
Macromolecular Substances / metabolism
Magnetic Resonance Imaging / methods*
Sonication / methods*
Tissue Distribution
Tissue Engineering / methods*
Grant Support
ID/Acronym/Agency:
AR046121/AR/NIAMS NIH HHS; AR056145/AR/NIAMS NIH HHS; R01 AR056145/AR/NIAMS NIH HHS; R01 EB000744/EB/NIBIB NIH HHS; R01 EB000744/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Macromolecular Substances
Comments/Corrections

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