| Magnetic resonance studies of macromolecular content in engineered cartilage treated with pulsed low-intensity ultrasound. | |
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MedLine Citation:
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PMID: 20807015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Noninvasive monitoring of matrix development in tissue-engineered cartilage constructs would permit ongoing assessment with the ability to modify culture conditions during development to optimize tissue characteristics. In this study, chondrocytes seeded in a collagen hydrogel were exposed for 20 min/day to pulsed low-intensity ultrasound (PLIUS) at 30 mWcm(-2) and cultured for up to 5 weeks. Biochemical assays, histology, immunohistochemistry, Fourier transform infrared spectroscopy, and magnetic resonance imaging (MRI) were performed at weeks 3 and 5 after initiation of growth. The noninvasive MRI measurements were correlated with those from the invasive studies. In particular, MRI transverse relaxation time (T2) and magnetization transfer rate (k(m)) correlated with macromolecular content, which was increased by application of PLIUS. This indicates the sensitivity of MR techniques to PLIUS-induced changes in matrix development, and highlights the potential for noninvasive assessment of the efficacy of anabolic interventions for engineered tissue. |
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Authors:
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Onyi N Irrechukwu; Ping-Chang Lin; Kate Fritton; Steve Doty; Nancy Pleshko; Richard G Spencer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural Date: 2010-10-25 |
Journal Detail:
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Title: Tissue engineering. Part A Volume: 17 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-31 Completed Date: 2011-05-02 Revised Date: 2012-04-13 |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
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Languages: eng Pagination: 407-15 Citation Subset: IM |
Affiliation:
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Magnetic Resonance Imaging and Spectroscopy Section, Gerontology Research Center 4D-06, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage, Articular / cytology, physiology*, radiation effects Cattle Cells, Cultured Chondrocytes / cytology, physiology*, radiation effects Chondrogenesis / physiology*, radiation effects Glycosaminoglycans / metabolism* Macromolecular Substances / metabolism Magnetic Resonance Imaging / methods* Sonication / methods* Tissue Distribution Tissue Engineering / methods* |
| Grant Support | |
ID/Acronym/Agency:
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AR046121/AR/NIAMS NIH HHS; AR056145/AR/NIAMS NIH HHS; R01 AR056145-03/AR/NIAMS NIH HHS; R01 AR056145-04/AR/NIAMS NIH HHS; R01 EB000744/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 0/Macromolecular Substances |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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