| Magnetic Levitation of MC3T3 Osteoblast Cells as a Ground-Based Simulation of Microgravity. | |
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MedLine Citation:
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PMID: 20052306 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Diamagnetic samples placed in a strong magnetic field and a magnetic field gradient experience a magnetic force. Stable magnetic levitation occurs when the magnetic force exactly counter balances the gravitational force. Under this condition, a diamagnetic sample is in a simulated microgravity environment. The purpose of this study is to explore if MC3T3-E1 osteoblastic cells can be grown in magnetically simulated hypo-g and hyper-g environments and determine if gene expression is differentially expressed under these conditions. The murine calvarial osteoblastic cell line, MC3T3-E1, grown on Cytodex-3 beads, were subjected to a net gravitational force of 0, 1 and 2 g in a 17 T superconducting magnet for 2 days. Microarray analysis of these cells indicated that gravitational stress leads to up and down regulation of hundreds of genes. The methodology of sustaining long-term magnetic levitation of biological systems are discussed. |
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Authors:
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Bruce E Hammer; Louis S Kidder; Philip C Williams; Wayne Wenzhong Xu |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Microgravity science and technology Volume: 21 ISSN: 0938-0108 ISO Abbreviation: - Publication Date: 2009 |
Date Detail:
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Created Date: 2010-1-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100971602 Medline TA: Microgravity Sci Technol Country: - |
Other Details:
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Languages: ENG Pagination: 311-318 Citation Subset: - |
Affiliation:
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Department of Radiology, University of Minnesota, 420 Delaware St., MMC 292, Minneapolis, MN 55455, USA, URL: www.ciamr.umn.edu. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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R21 EB003947-01A2//NIBIB NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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