| Magnetic Glyconanoparticles as a Versatile Platform for Selective Immunolabeling and Imaging of Cells. | |
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MedLine Citation:
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PMID: 21247095 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A versatile nanoplatform based on magnetic glyconanoparticles (glyco-ferrites) to attach well-oriented antibodies is described. An efficient ligand exchange process has been used to prepare water-soluble 6-nm-sized core-shell Fe(3)O(4)@Au nanoparticles bearing amphiphilic carbohydrates and aliphatic ethylene glycol chains ended in a carboxyl group. The covalent immobilization through the carboxyl group of an Fc receptor (protein G) enables successful well-oriented capture of immunoglobulins G onto the magnetic glyconanoparticle. A thorough characterization of structure and biofunctionality of the constructs is carried out by different techniques. The selective immunolabeling of cells by the antibody-magnetic glyconanoparticle conjugates is demonstrated by magnetic resonance imaging (MRI), as well as by fluorescence techniques. |
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Authors:
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Isabel García; Juan Gallo; Nuria Genicio; Daniel Padro; Soledad Penadés |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-19 |
Journal Detail:
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Title: Bioconjugate chemistry Volume: - ISSN: 1520-4812 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Laboratory of GlycoNanotechnology, Biofunctional Nanomaterials Unit, CICbiomaGUNE and CIBER-BBN , P° de Miramón 182, 20009 San Sebastian, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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