| Magnetic Fe₃O₄ nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells. | |
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MedLine Citation:
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PMID: 21187919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to investigate the potential effects of combination therapy using magnetic nanoparticles of Fe₃O₄ (MNP-Fe₃O₄) and chemotherapeutic drugs on lymphoma cells. Proliferation, inhibition, and viability of Raji cells were detected by MTT and trypan blue exclusion. The percentage of cells undergoing apoptosis was detected by flow cytometry using fluorescein isothiocyanate-annexin V and propidium iodide staining. p53 and nuclear factor-κB (NF-κB) protein levels were measured by Western blot. The results showed that proliferation of Raji cells was inhibited by adriamycin or daunorubicin in a dose-and time-dependent manner. Cell sensitivity was improved and the 50% inhibitory concentrations of adriamycin and daunorubicin decreased when combined with a MNP-Fe₃O₄ carrier. Interestingly, increased apoptosis in Raji lymphoma cells was accompanied by upregulation of p53 protein and downregulation of NF-κB protein. Furthermore, the combination of MNP-Fe₃O₄ with adriamycin or daunorubicin increased p53 protein levels and decreased NF-κB protein levels more than adriamycin or daunorubicin alone, indicating that MNP-Fe₃O₄ could enhance the effect of chemotherapeutic drugs on p53 and NF-κB. Similar results for cell apoptosis and protein expression were not observed for the groups treated with dexamethasone ± MNP-Fe₃O₄ (P > 0.05). These findings suggest a potential clinical application for MNP-Fe₃O₄ in combination with daunorubicin or adriamycin in the treatment of lymphoma. |
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Authors:
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Hongmei Jing; Jing Wang; Ping Yang; Xiaoyan Ke; Guohua Xia; Baoan Chen |
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Publication Detail:
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Type: Journal Article Date: 2010-11-19 |
Journal Detail:
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Title: International journal of nanomedicine Volume: 5 ISSN: 1178-2013 ISO Abbreviation: Int J Nanomedicine Publication Date: 2010 |
Date Detail:
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Created Date: 2010-12-28 Completed Date: 2011-04-18 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 101263847 Medline TA: Int J Nanomedicine Country: New Zealand |
Other Details:
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Languages: eng Pagination: 999-1004 Citation Subset: IM |
Affiliation:
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Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Beijing, People's Republic of China. jinghm@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibiotics, Antineoplastic
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pharmacology* Antineoplastic Combined Chemotherapy Protocols / pharmacology* Apoptosis / drug effects* Blotting, Western Burkitt Lymphoma / drug therapy*, metabolism, pathology Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Daunorubicin / pharmacology Doxorubicin / pharmacology Drug Synergism Flow Cytometry Humans Magnetite Nanoparticles / chemistry* NF-kappa B / metabolism Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Magnetite Nanoparticles; 0/NF-kappa B; 0/TP53 protein, human; 0/Tumor Suppressor Protein p53; 20830-81-3/Daunorubicin; 23214-92-8/Doxorubicin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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